| Objective: To investigate the effects of calcitonin gene related peptide(CGRP) on the human hair growth in vitro. Method: Normal human scalp samples were obtained, and anagen hair follicles and dermal papilla cells were isolated cultured. The culture anagen hair follicles were divided into two groups: a control group and a CGRP(10-7 M) group. The results were evaluated by measuring linear hair growth and hair follicle morphology under a light microscope. In addition, after pretreatment of cultured hair follicles and dermal papilla cells with CGRP(10-7M), we examined the changes of expression of hair growth factors(FGF-7, IGF-1, VEGF), hair growth-inhibitory factor(IL-1α, IL-1β) and apoptosis-related molecules(P53, caspase-3). Results: The CGRP did not have statistically significant effect on the rate of linear hair growth in cultured hair follicles. However, it shortened the anagen stage of the hair cycle. In hair follicles, the expression of IL-1β, a hair growth inhibitory factor, was increased than control. In dermal papilla cells, the expression of IL-1β was increased than control. Conclusion: CGRP shorten the duration of the anagen phase of the hair growth cycle, and this effect probably occurs at least in part via up regulation of the recognized hair growth inhibitory factor IL-1beta. |
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