Gynostemma Extract Light Aging Model Mice Skin Tissue Cat, The Ghs-px Activity And The H <sub> 2 </ Sub> O <sub> 2 </ Sub> Content Of The Experimental Research

Objective: To investigate G. Pentaphyllum' s influence on the activity of Glutathione peroxidase (GHS-PX) and Catalase (CAT) in the skin of hireless photoageing mice as well as the content of hydrogen peroxide (H₂O₂) in their skin, and to investigate G. Pentaphyllum' s effect on the structure of the hireless photoageing mice.Methods: Healthy KM hireless mice were divided into five groups randomly and gavaged respectively, that is negative Control Group (A), Model Group (B), Low- G. Pentaphyllum -Dosage Group (C), Medium-G. Pentaphyllum -Dosage Group (D), High- G. Pentaphyllum -Dosage Group (E). The mice in B, C, Dand E group were irradiated to simulate skin photoageing by long-term ultraviolet irradiation, at the same time, group B were given vitamin E while those in C, D, and E were given G. Pentaphyllum. Staining skin with HE, Under 40 times light microscope, the structure of dermal was observed. While the changes of the content of H₂O₂, as well as the activity of GHS-PX and CAT in the skin were measured by biochemical test method in each group of the experiment.Results: In model group, the activity of GHS-PX and CAT were decreased , while the content of the H₂O₂ was increased seriously (P <0. 01) .The skin biopsies appear photoageing look. Compared with the group B, the activity of CAT in group D was made a great progress (P <0. 01) ; as well as the activity of GHS-PX was increased and the content of H₂O₂ was reduced in dermal in group C and E, furthermore the latter is dependent on dosage obviously (P<0. 01, P<0. 05) .Conclusions: G. Pentaphyllum has the protective effect on the skin of the photoageing hairless mice. The mechanism is that on one hand, G. Pentaphyllum could eliminate free radicals itself; on the other hand, by increasing the activity of GHS-PX and CAT, it could inhibit the oxidative damages of ultraviolet and skin photoaging.