Drinkers Music On Chronic Pancreatitis In Rats, Pdgf-bb And Fn Expression

Objective:To study the effects of Chinese herbs Jiu-Ke-Le ( JKL ) on platelet-derived growth factor-BB ( PDGF-BB ) and Fibronectin ( FN ) in pancreatic tissues in experimental animals and , along with the relationship between alcohol-drinking and diabetes mellitus which is complicated by CP, we establish the rat model of chronic pancreatitis (CP). This research tried to study and analyse the mechanism of preventing and treating CP. Methods:Male Wistar rats weighing 180-210g were randomly divided into 6 groups: Group A : JKL intragastric administration treatment group, Group B: pathology model group, Group C: Daji treatment group, Group D: normal control group, Group E, Group F(to study the relationship between alcohol-drinking and diabetes mellitus, group E and group F were given intraperitoneal injection of DDC and were perfused stomach with alcohol. Group E offered JKL while Group F did not ). Each group had 16 rats. Group E and Group F were perfused stomach with alcohol of 8g/kg every day. Except group D, the other rats were used as pathology models of CP. They were induced by intraperitoneal injection of 750mg/kg body weight (BW) of diethyldithiocarbamate (DDC) twice a week for 4 weeks. During the experiment periods, the appetite, water volume, urine volume, stool character should be observed, and weigh the rats once a week. After 4 weeks, the control group and the pathology model one were perfused stomach with saline, 10ml/kg BW. JKL intragastric administration treatment group 10ml/kg BW(2g/kg) and Daji treatment group lOml/kg BW(50mg/kg), once a day, lasting 4 weeks. The day before the last day, the weight of all the rats were noted. Then fasting 24 hours, noting the blood glouse, sampling blood in rat's eyeball .Immediately the rats were put to death. Then got out the rats pancreas for the research of immunohistochemistry to measure the expression of PDGF-BB and FN and for the observation by tramission electron microscope. Results:The CP rats' common conditions of group A, C, E were obviously better than that of model group B. The body weight of group A, E were better than than that of model group B (P<0. 05) . The levels of blood glucose of A, E were lower than that of model group B (P<0. 05 ) . The levels of amylase of A, E were lower than that of model group B (P<0. 05) . MYS was able to decrease the expression of platelet-derived growth factor-BB and Fibronectin in pancreatic tissues (P<0. 05 ) . Conclusion:The models of chronic pancreatitis rats can be induced within 4 weeks by intraperitoneal injection of DDC twice a week. JKL can not only improve...