Myasthenia Gravis Passive Transfer Of Young Rats Mucosal Immune Tolerance

PARTⅠINDUCE A MODEL OF PASSIVE TRANSFERRED MYASTHENIA GRAVIS ON YOUNG MICEObjective: To induce an animal model of passive transferred myasthenia gravis (PTMG) on young C57BL/6 mice with different doses of nicotinic acetylcholine receptor monoclonal antibody-mAb35, to decide the effective dose of mAb35 required to induce PTMG, and to provide an animal model for further study of the pathogenesis and immunotherapy of human MG.Methods: The young C57BL/6 mice in the experimental groups were divided into three subgroups (E1, E2, E3), injected intraperitonealy (i.p.) with 0.2 ml of Ringer's buffer solution containing 0.5, 1.0 or 1.5 mg/kg of capital monoclonal antibody mAb35 of acetylcholine receptor respectively. The control group (N) were i.p. injected with 0.2 ml of Ringer's buffer solution with no mAb35. In order to decide whether the model was a success, the clinical symptoms of the mice were observed, evaluation experiments on pharmacology were done with neostigmine methlsulfate, repetitive nerve stimulation (RNS) was carried out, the serum level of acetylcholine receptor antibody (AChRAb) was detected by ELISA, and the ultrastructure of end plates was observed with acetylcholinsterase...