Through Nine Stagnation Capsule Of Rats Following Chronic Stress And Mechanism Study

Objective: To provide the theory for clinical medication, we observed the effect of TONGJIUJIEYU capsul on chronic stress rat model of depression and explored the mechanism of it.METHODS: Male Sprague-Dawlet rats were housed in draughty conditions with natural illumination, with access to food, water ad libitum,and were allowed to acclimate to the housing conditions for 7 days.Open-field test was produced before experiments began. According to the result of Open-field test and the weight of each rat, the animals which have the similar scores were choosed to be a group and all of them were divided into 4 groups of 10 each.(1) Normal group was given distilled water (2ml per rat, i.g.) once per day;(2) Model group was given distilled water (2ml per rat, i.g.) once per day; (3)Group of traditional Chinese drug was given TONGJIUJIEYU capsul(2ml per rat,i.g.) once per day, the dose of which equals crude drug of 216mg/kg in 2ml distilled water;(4)Positive control group was given fluoxertine hydrochloride (i.g.) 7.2mg/kg in distilled water of 2ml.Drugs were chronically administered to animals at AM 9:00～11:00 for 21 days.We formed the chronic stress rat model of depression by singly housing and exposing to unpredictable gentle stressors and did the following experiments:Evaluate the antidepressant effect by Open-field test and the weight of rats; Detect the concentration of NE,5-HT,DA in the blood serum by chemiluminescence's assay and explore the mechanism of antidepressant effect; To observe the mechanism of immunoloregulation of TONGJIUJIEYU capsul, we detected the inflammatory...