| Nanomaterials have been used for several decades in biomedical field. Some nanomaterials are metabolizable while others are bio-inert. ACNP, Indian ink and single wall nanotube (SWNT) are all bio-inert. They can not be metabolized. To study the biocompatibility of bio-inert nano-particles in vivo, nano-particles were intraperitoneally or intravenously injected into mice to observe the distribution, migration and excretion of nano-particles. To explore biological toxicity of SWNT, the SWNT without any chemical modification was administered through gastrointestinal tract by gastrogavage in a dose of 50 mg/kg , 100 mg/kg, 150 mg/kg per day for 10 days.Experiments with ACNP intraperitoneal administration show that ACNP have obvious lymph-tissue-targeting effects. The lymph-targeting effect is obtained by the diameter of ACNP. Anatomic and pathological examinations of the mice showed no exudation, edema, organization and encapsulaton in peritoneal tissues and internal organs. There were no inflammation and conglutination in peritoneal cavity. These results showed that ACNP have good biocompatibility with animal tissues and low toxicity to system as a drug carrier.The experiments with intravenous ACNP showed that they can be excreted by some ways, such as biliary canal, urinary duct and the goblet cells in intestinal epithelium. It is the first time that we report that bio-inert nano-particles can be excreted from the animal body. These results further consolidated that ACNP is a safe drug carrier possessing good promises.The SWNT has potential wide uses and may be expected to be massively produced in not too long a time but SWNT is also possibly harmful to animal and human as a material with nanometer sizes. For the safe usage of SWNT, the biocompatibility of the naked single wall nanotube (NSWNT) has been studied in mice. The SWNT without any chemical modification was administered through gastrointestinal tract by gastrogavage in a dose of 150 mg/kg per day for 10 days. |
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