The Xnji Brain Injury In Rats, Apoptosis And Related Protein Expression

ObjectionTraumatic brain injury is a kind of severe brain tissue damage that has high fatality rate and mutilate rate. In brain injury mechanism secondary brain injury have the significant role and conclude patient's prognosis. Large empirical study have confirmed that apoptosis was the significant cell death form in secondary brain injury. The essential component of XingNaoJing injection include musk, borneol, gardenia and tuber et al. XingNaoJing injection have began to treat brain injury and have curative effect in recent years. The topic observe the effects of Xingnaojing injection on the cell apoptosis and the expression of apoptosis - regulatory proteins (including Bcl - 2 , Bax and caspase - 3) in rats after traumatic brain injury ( TBI) and to investigate the effect and mechanism of Xingnaojing on apoptosis ,MethodsOne hundred-eight common male Wistar rats were randomly divided into three groups , including sham - operated group, only TBI group and Xingnaojing treatment after TBI group. Every group include thirty - six and divided into 6h,12 h, 24 h, 48 h, 72 h and 140 h groups after TBI and each include six rats. Sham-operated group do craniotomy only, another groups establish the animal model of TBI by modified Feeney's method. Xingnaojing treatment after TBI group were intraperitoneal injected XingNaoJing injection 20ml/kg after TBI 10 minutes only daily . Only TBI group was intraperitoneal injected sodium chloride solution 20ml/kg after TBI 10 minutes only daily. Rats were executed by decapitation and dislodged brain tissue which be fixed by put in neutral formalin solution . Terminal - deoxynucleotidyl transferase mediated biotin-dUTP nick end labeling(TUNEL) was used for apoptosis detection and immuno - histochemical staining was used to detect the expression of Bax , Bcl - 2 and Caspase - 3 proteinum. To observe immunoreactive effect of TUNEL , Bel - 2, Bax and caspase-3 by 400 HP light microscope and compute average masculine cell population per example randomly five eye-reach.ResultsThe masculine expression of Bcl - 2, Bax, Caspase - 3 protein mainly in endochylema or caryotheca and show diffuse or scatter buffy particle diameter. Bcl-2 protein's expression decreased from 6h after TBI and achieve the lowest at 3 days after TBI. The expression of Bcl-2 protein of Xingnaojing treatment after TBI group decreased have no significant change in every time spot and have statistical significance after 24h ( P<0.05 ) . The expression of Bax protein iecreased from 6h after TBI and achieve the highest at 2-3 days after TBI; The expression of Bax protein of Xingnaojing treatment after TBI group increased lower than only TBI group and have statistical significance after 72h ( P<0.05 ) . The expression of casepase-3 protein increased from 24h after TBI and achieve the highest at 3 days after TBI; The expression of casepase-3 protein of Xingnaojing treatment after TBI group increased lower than only TBI group and have statistical significance after 24h ( P<0.05 ). The masculine expression of TUNEL mainly in cell nucleus and show buffy particle diameter. The masculine expression of TUNEL could be find seldom in normal brain tissue and increased from 6h after TBI and achieve the highest at 3 days after TBI; The expression of TUNEL of Xingnaojing treatment after TBI group increased low than only TBI group and have statistical significance after 48h...