Bladder Transitional Cell Carcinoma Dbccr1 Of P16 And Of P15, P14 Gene 5'cpg Island Methylation Analysis

Objective To evaluate the methylation status of 5'CpG island of DBCCR1, P15, P14 gene and their significance. Methods The methylation status of 5'CpG island of DBCCR1, P16, P15, P14 gene were examined in 40 cases of BTCC and 8 cases of normal urothelium by means of methylation-specific PCR.x~2 test was used to assess the relationship between the degree of methylation tumor grade and stage.Results The degree of methylation of DBCCR1, P16, P15, P14 gene were found 42.5%, 21.5%, 17.5%, 35% respectively in cancer tissues and 0% in normal urothelium. The significant difference of methylation was found in both P16 and P14,but it was't found in DBCCR1 and pl5.Conclusions The degree of methylation of 5'CpG island of DBCCR1, P16, P15, P14 gene were higher than that in normal bladder mucosa. The Aberrant hypermethylation of four tumor suppressor genes at chromosomal 9 played an important role in the occurrence and development of bladder tumor. The hypermethylation of 5'CpG island of DBCCR1 , P15 gene were an important event in the bladder tumor(P<0.05). However, The hypermethylation of P16,P14 gene were a late event (P>0.05) .