The Trollius Protein Identification Characteristics And Activity

Flos Trollii Chinensis is the dryness flower of Trollius chinensis Bunge. It is one of higher valuable antimicrobial Chinese traditional medicine. Modern pharmacology research proves: Flos Trollii Chinensis has widely antimicrobial spectrum. It has been widely used in treating respiratory and gastrointestinal infections.Based on quantitative pharmacology and molecular biology technologies, we can objectively reflect Chinese medicinal value or give out other indicators of genetic characteristics of "biological identification method" as one of the advanced methods for evaluating the quality of Chinese traditional medicines.The experiment established the Flos Trollii Chinensis protein electrophoresis testing SDS-PAGE system, studied in protein levels of different regions and different collection time samples, which will bring some information for further investigation of Flos Trollii Chinensis. The primitive anti-microbial activity of Flos Trollii Chinensis protein has been investigated, which will direct for the exploitation of Flos Trollii Chinensis.The main results in this experiment were shown as follows:1 The extraction of Flos Trollii Chinensis protein.Base on studying and comparing with other methods,the L9(34) orthogonal test has been used to optimize the extraction method. The optimized ultrasonic method was as follows: powdering 60 mu, adding 30 times water, extracting for 30 minutes.2 The establishment of the SDS-PAGE experimental system for Flos Trollii Chinensis protein.The experiment condition shows as: the separation gel's concentration is 12%, the concentrate gel's concentration is 5%, the sample's quantity is 20μL, and the electrophoresis time last for about 2.5hours.By using the best dyeing method to treat the gel, and the clear protein strips with hypochromic background had been acquired. The gradient gel (5%-20%) has been attempted to separate the protein.The experiment analysis showed: By using SDS-PAGE system the total Flos Trollii Chinensis protein can be separated to 10-14 components, which are 10KD,14KD,17KD,21KD,30KD,33KD,39KD,41KD,45KD,53KD,60KD,64KD,70KD,81KD. Among them, the sample 1 to 7 and sample 9 have 4 primal grade bands(70KD,45KD,41KD,33KD), and 4 secondary grade...