| Neuroglobin (Ngb), an oxygen-carrying protein mainly present in neurons of the brain, plays a very important part in the process of ingestion, transportation and utilization of oxygen. It is supposed to act as an endogenous neuroprotective factor against ischemic damage. Retina is one of the highest oxygen-consumption tissues in human body, and more and more medical workers pay attention to the relationship between Ngb and ocular ischemia.We set up the animal model of ischemical reperfusion injury in accordance with the hypothesis that there is a close relationship between NGB and ischemic damage and the therapeutic action of Dangguibuxue decoction. And we aimed to explore the change of NGB expression, explain their relationship, and certify the preventive and therapeutic effect of Dangguibuxue decoction.Objective:1. To establish the model of acute ischemical reperfusion injury in rats, confirm the changes of the NGB mRNA in retina under the pathologic state, and discuss the relationship between NGB and acute ischemical reperfusion injury.2. To discuss the prevention of Dangguibuxue decoction to acute ischemical reperfusion injury.Material and Methods:Our study was carried out in adult male Sprague-Dawley rat(200g±10g). We separated the oculorotary muscles to exposure thoroughly the neural sheath of optic nerve, then separated the optic nerve, clamped partly central artery and vein the distance of 1.5 mm from discus opticus with blood vessel forceps, and finally undamped the blood vessel forceps. The rats were divided into 3 big groups randomly (model group, Chinese medicine group, Sibelium group ), 12 small groups(according to the time of drawing the materials afteroperation, each big group was divided into 4 small groups ), normal group ,and sham operation group. We determined the expression of NGB gene using real time-polymerase chain reaction (RT-PCR). We used one-factor analysis of variance to draw a conclusion for the data process and statistical analysis.Results:1. The weight of the rats in Chinese medicine group (306.00±20.69g) rose remarkably (PO.01).2. The "A" (NGB/GAPDH) of the first 3 small group in model group rose sharply (the "A" of them were 1.43733±0.288874, 1.24350±0.258606, 1.12233±0.247122), and the discrepancy between model group and normal group(0.50750±0.036594) was very remarkable(P<0.01). The "A" between the last small group(0.57867±0.108286) and normal group had no discrepancy (P>0.05).3. The discrepancy between the first 3 small group in Chinese medicine group (the "A" of them were 0.88433±0.191541, 0.78933±0.088065, 0.56217±0.077295) and model group was very remarkable (P<0.01), and the "A" between the last small group in Chinese medicine group (0.52083±0.043009) and normal group had no discrepancy(P>0.05).4. The discrepancy between the first 2 small group in Chinese medicine group and normal group was very remarkable (P<0.01), and the "A" between the last 2 small group in Chinese medicine group and normal group had no discrepancy(P>0.05).5. The A between Sibelium group and model group had no discrepancy (P>0.05).6. The A between normal group and sham operation group (0.50500±0.047590) had no discrepancy (P>0.05).Conclusions:1. It was successful to set up the model of acute ischemical reperfusion injury.2. The expression of NGB gene rose sharply during the earlier period of acute ischemical reperfusion injury.3. Dangguibuxue decoction promoted the recovery of the expression of NGB gene during the earlier period of acute ischemical reperfusion injury, and protected retina from injury.4. Sibelium had no effect found on acute ischemical reperfusion injury. |
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