Tetramethylpyrazine, Taurine Hyperoxic Lung Injury

Objective: To study the effects of TMP and Tau on apoptosis index of neonatal rats lungs with hyperoxia damage.Methods: Select 2-day old, 5-10g, 160 newborn rats to devide them into 8 groups: Ⅰ 3days normal control group , Ⅱ 7days normal control group , Ⅲ 3days hyperoxia group. Ⅳ 7days hyperoxia group, Ⅴ 3days hyperoxia+TMP group ,Ⅵ 7days hyperoxia+TMP group, Ⅶ 3days hyperoxia+Tau group , Ⅷ 7days hyperoxia+Tau group .The newborn rats begin to be interaperitoneally injected with TMP(30mg/kg) and Tau(200mg/kg) after being established hyperoxia-induced lung injury model for 2days, then the rats are killed after hyperoxia for 3days and 7days to exam the lung histophathological changes stained Dy HE and TUNEL, to detect the apoptosis index of alveolar wall cells. For parts of rats, taken broncho-alveolar lavage fluid to detect total protein and MDA levels, compare the conclusions into normal control group ,hyperoxia group and drug group.Results: The apoptosis indexes of hyperoxia groups were much higher than that in normal control groups(p<0.01). The apoptosis indexes of drug groups were much lower than that in saline groups(p<0.01). The total protein and MDA levels in BALF of hyperoxia groups were much higher than that in normal control groups(p<0.01). The total protein and MDA levels in BALF of drug groups were much lower than that insaline groups(p<0.01).Conclusion: TMP and Tau can decrease cerebrocellular apoptosis in lung tissues with hyperoxia damage. It also can decrease total protein and MDA levels in BALF of neonatal rats lung with hyperoxia damage, so TMA and Tau have prevention and cure effect on neonatal rats s lung with hyperoxia damage.