Nitric Oxide Synthase Expression In Rats With Diabetes Colon Dysfunction Of The Colon Wall And The Significance Of The Ap-1 Fine Wine Striatal Synaptic Plasticity Change Impacts

Colonic dysfunction is a frequent complication of diabetes mellitus, and constipation is the most common clinical situation. Even though autonomic neuropathy , gastrointestinal hormone and vasodilation dysfunction are regarded as the important mechanisms of Colonic dysfunction, it requires further clarification..It has been demonstrated that Nitric oxide (NO) is a simple and unique molecule that can serve many diverse functions, including those as an ntracellular second messenger and intercellular messenger.NO is produced from 1-arginine by the activation of NO synthase (NOS). There are three isoforms of NOS that are products of distinct genes. They are Neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS).NO and its synthesizing enzyme NOS seem to be involved in colonic dysfunctiono In this study we investigated the expression of three subtypes of NOS in the colon of diabetic rats, and to explore the relation between colonic dysfunction and NOS expression.Sprague-Dawley (SD) rats were used in this experiment. Diabetic rats were induced by streptozotocin (100mg/kg, i.v.). Three isozymes of NOS (nNOS, iNOS and eNOS) expression in proximal and distal colon were measured in 4 weeks after diabetes induction through the methods of immunocytochemistry and semi-quantitative reverse...