Ginsenoside Rb1 On The Protective Effect And Mechanism Of Neonatal Rat Cardiac Myocytes To Hydrogen Peroxide Injury

Ischemia-reperfusion injury is one of the difficult problems facing the curing procedure of ischemic heart disease. The traditional Chinese medicines are now getting more and more attention for their unique effect. Ginsenoside, one of the major components of gen-seng, has been studied for many aspects. However, most of these studies have been done using the entire animal model, which made it easily effected by many other factors in vivo. In order to investigate the anti-reperfusion effect as well as the possible mechanism of ginsenoside Rbl to cardiomyocytes, hydrogen peroxide (H₂O₂) of 100μmol/L, which is close to the real concentration in the ischemia-reperfusion injury, was used on the cultured neonatal rat cardiomyocytes.This study evaluated the protective effect of ginsenoside Rbl to cardiomyocytes by investing the leackage of lactic acid (LDH), cell apoptosis and the metabolism activity of the cardiomyocytes treated by H₂O₂. The result showed that ginsenoside Rbl decreased the LDH leakage and the apoptosis rate, and increased the metabolism activity of the cardiomyocytes These results revealed that ginsenoside Rbl had protective effect on the cardiomyocytes against H₂O₂ injury.To understand the mechanism of the protective effect of ginsenoside Rbl, the malondialdehyde (MDA), superoxide dismutase (SOD), the intracelluar calcium, as well as the expression of Fas and Bc1-2, was measured in the cardiomyocytes after treated by H₂O₂. The result showed that ginsenoside Rbl decreased the MDA and increased the activity of SOD. Also it decreaesd the intracellular calcium overload. These could be the mechanisms leading to the protective effect of gensenoside Rbl agains H₂O₂ injury.