| Hydrocephalus is a multifactorial brain disorder characterized by ventricular dilation and increase of overall CSF levels due to either increased production or decreased absorption of CSF. Modern medicine is short of effective treatment on the disease. Since thousands years ago ,There are many methods in TCM to relieve hydrocephalus and protect intellect and mitigate sequela. This paper focus on the pathogenesis of hydrocephalus caused by TGF-β1, and divided into two parts, namely, the document research and experimental research. The document research include: the role of TGF-β1 in the pathogenesis of hydrocephalus, the therapy of hydrocephalus in traditional Chinese medicine. In the experimental research part, we used the method of intrathecal injection of human recombinant transforming growth factor-beta 1(TGF-β1) in 7-day-old rats. ①Observing the change of morphology of neuron in the model at the different time point after injection 3d, 7d, 2w and 4w. Finding out the possible mechanism of Nao Kang ling (a Chinese medicine ) treating hydrocephalus by comparing the model group and the therapy group. ② Observing the action of all groups at 4weeks later after therapy to judge the destroy of intellect and the effect of NAOKANGLING. The result showed: 1. The Morphology of neuron The main character was the inflammation of brain tissue which led to ventricular delation, cortex atrophy, dead and putrescence of neuron in hippocampus in the acute period of this hydrocephalus model. 2. The influence of NAOKANGLING on synaptophysin Immunohistochemical finding of synaptophysin showed that the density mean of model group was significantly lower than normal group at the 3rd day after treatment, yet the density mean of other groups in different time points were higher than normal groups with significant difference. After treating 2 weeks, different dosage of NAOKANGLING treating groups had not obvious difference with normal group already, as well as 4 weeks after treating. 3. The influence of NaoKang-ling on neuron apoptosis The result showed that the number of neuron apoptosis in model group was obviously more than that in normal group and treating groups. There was no difference between NAOKANGLING treating groups and normal group or NaoFu-kang treating group. 4. The influence of NAOKANGLING on neuron regeneration The immunohistochemical finding showed that few regenerative cells appeared on ependyma in normal group, yet plenty of cells with brown nucleolus could be seen on ependyma in treating groups both NaoKang-ling and NAOFUKANG.In model group, regenerative cells also could be found. 5. The influence of NAOKANGLING on the ability of study and memory It was found in the experiment that the time of staying on the platform in model group was less than that in normal group and treating groups. There was no difference between NAOKANGLING treating groups and NaoFu-kang treating groups, as well as normal group. 6. The influence of NAOKANGLING on spatial learning ability Hydrocephalus induced by TGF-β1 can destroy spatial learning ability in rats. In our experiment, the time to find out the platform in model group was obviously extended, which possibly related with the elevatory titer of TGF-β1 in CSF inducing subarachnoid fibrosis. It could be significantly shortened through treating with NAOKANGLING for 4 weeks, which showed that NAOKANGLING had the effect to alleviate the trauma of neuron and clinical seqeula. A rat model of hydrocephalus can be founded by intrathecal injection of human recombinant transforming growth factor-beta 1(TGF-β1).It suggested that NAOKANGLING has curative effect to the disease through analysis of morphology and behavior after treating. TGF-β1 plays a critical role in the pathogenesis of hydrocephalus. The weight of animal model almost stop increasing 4 weeks later after treatment .Skull inflating appeared in several animals, which were found thinner osseous, attenuation of pallium, encephalon atrophy and hydrops under leptomeninx. The study of morphology showed that there w... |
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