The Brain Stem Drink Game Sexual Stove Cerebral Ischemia-reperfusion Injury In Experimental Research

Objictive: To measure the protective effects of naogengying on rat brain tissue from the area of cerebral ischemia — reperfusion injury in rat and continue to investigate its mechanisms.Method: the model of focal reperfusion injury after cerebral ischemia in rats was made by middle cerebral artery occlusion. The effect of naogengying on express ion of bcl — 2 proin in rats brain tissue were observed by immunohistochemical method during different time of cerebral ischemia—reperfusion. TTC staining and HE staining was applied to observe cerebral infarct volume and pathology change in brain tissue respectively. Zea Longa method was use to evalue behavioral change.Result: Compared with the control groups. Zea Longa neurological grades intreated- naogengying group were lower than those in control. there were great difference between the control groups and groups with naogengying. (P<0.05) .The infact area was 24.37% for treated rats vs 36.12%for control rats. .The volume of cerebral infarction was decreased 40% in treated groups. The expression of bcl—2 proin were not find in normal groups. Both control and treated groups detected bcl —2 expression at 6 hour 12 hour and 24 hour after reperfusion . bcl—2 expression of the treated groups were more increased than that of the control groups in each hour point .more much at 12 hour. there were great difference between the control groups and treated groups in expression of bcl—2 proin(P<0.05). the treated -groups diminished damage to brain tissue.Conclusion: naogengying has great cerebral protective effects against ischemia—reperfusion injury by increasing restrain mechanisms of the apoptosis of neuronal cell and expression of bcl—2 protein , reduced the volume of cerebral infarction and damage to brain tissue.