Houdain Pill Improved The Rat Endometriosis Model

In recent years, the increasing incidence of endometriosis(EMS) has been attacting more and more attention of medical scholars. Traditional Chinese medicine is one of the main therapy of EMS, and compared with Western medicine, it has advantages of less side-effects, lower relapse rate, permitting longer use and stable curative effect,et al. The WuDan Pill(WDP) is a self-made medicine by the DongFang Hospital of the BeiJing University of TCM. It has been used in clinic for decades and showed prominent effect. But the large prescription of WDP composing 22 spices has limited the preparation technics and quality standard control, and on the other hand, the WDP has not been refined, so it is difficult for patients to digest and even causes stomachache after taking it. Our task is to meliorate the presciption of WDP to 9 spices composing red teony root, red sage root, cassia bark, corydalis tuber, dyied leech, centipede, processing fresh-water turtle shell, sappan caesalpinia, zedoary. We have studied the therapeutic effect of the Predigestion of WDP(PWDP) and WDP on the experimental induction of EMS in SD mouse before the clinic use of PWDP. By imitating an EMS model of SD mouse, we come to the following conclusions: the operators must be experienced enough to lessen the bleeding and infection of mice to the largest extent in order to increase the survival rate; we should sew up the uterus tissue close to the big veins so that the ectopic endometrium can grow well; to transplant the uterus segment close to the uterus corner was good enough to get a high success rate of model; to transplant the whole uterus segment to abdominal cavity rather than to apart the inner layer and the flesh layer of the endometrium may come to a successful model and predigest operation at the same time. After medication to the EMS model of mice, we concluded: the PWDP had favourable effect on the EMS model of mice, and although it had only 9 spices, it showed the same effects as WDP on the aspects of alleviating inflammation of the abdominal cavity, lessening the ectopic focus and accelerating the corrupt of the ectopic endometrium; the middle dose of the PWDP showed the best effects. Next, we will go on to study the curative mechanism and the clinic prospect of the PWDP.