| ObjectiveCerebrovascular disease(CVD) had become hazardous to the mankind, which was one of the severe diseases especially to the mid-old people. CVD was the first common disabling and the second common fatal disease in the globe. The attack rate of ischemic CVD was higher than that of hemorrhagic CVD, so investigating and treating the ischemic brain injurying was the focal point of preclinical medicine and clinical medicine. With the profoundly investigating the ischemic stroke, cerebral ischemic reperfusion injury was found when the bloodstream revived recanalization. Ischemical reperfusion injury was an intricated pathology and physiology course. The proper treatments to protect brain might elevate the tol of brain tissue to ischemic injury. Electro-acupuncture had been applied in treating cerebral ischemia diseases, and its therapeutical effects had been confirmed by generous clinical and empirical studies. This investigation studied the influence of electro-acupuncture for Dazhui and Neiguan on apoptosis and insulin-like growth factor-1 (IGF—1) of cerebral ischemic rats so as to provide the theoretical basis for acupuncture in the clinical treatment of ischemic cerebrovascular disease. MethodsAdopting 40 SD rats, weighing 200±20g, divided into four groups according to random digits table, 10 rats every group. Sham operation group, model(ischemia 0.5h reperfusion 48 hours), electroacupuncture treatment(ischemia 0.5h reperfusion 48 hours, treated the first time after ischemia 0. 5h reperfusion 3 hours , then treated another time every 12 hours) and the normal control group. Used the suture in MCA to establish MCAO ischemia-reperfusing model. The remarks of nerve function deficit referring to Lundy remarking standards. The acupoints of DAZHUI andNEIGUAN were selected, stimulated with continuous wave for 30 minutes, 120Hz/min, 1mA intensity. The rats were reperfused with 4 % paraformaldehyde after being anesthetized by 10% chloral hydrate, then taken out the brain. The brain tissue 2mm away from the chiasma was cut frontally, the middle part left, immersed in hard paraffin. Used immunohistochemistry SABC(spreptavidin-biotin-peroxidase complex) method to investigate the expression of IGF—1 and TUNLE staining. In the 40 times objects glass, IGF—1 positive reaction cells were of analysis of accounts. All the datum were processed by SPSS11. 0 statistics software package and all results were demonstrated by mean±standard deviation. Results1. The apoptosis cells were an extremely small quantity in normal group and sham operated group, while a large quantity of apoptosis cells of model group were spreading the whole CA1, the average ratio of apoptosis cells 92 + 10.51. There was highly significant deviation contrast to normal group and sham operated group (P<0. 01) . The more apoptosis cells were observed in CA1 in the electroacupuncture group, the average ratio of apoptosis cells 36±4.72 , There was highly significant deviation contrast to normal group (P<0. 01) . Electroacupuncture could decrease neurocyte cells apoptosis in rats' hippocampus of the cerebral ischemical reperfusion injury. 3. In the ischemia + electroacupuncture group, the quantity and intensity of IGF—1 immune positive expression were clearly increased than those in the other three groups (p<0.01).2.A small quantity weakly positive IGF—1 cell were observed in CA3 of normal group and sham operated group, those of model group were slightly increasing, and kytoplasm staining were light-midrangly positive. There was statistical significance compared to normal group and sham operated group ( P<0. 05 ) . After electroacupuncture treatment, positive IGF—1 cells increased, and kytoplasm staining were midrange-strongly positive. Therewas an significant difference compared to model group (P<0.01). ConclusionElectroacupuncture functions perhaps up-regulated the expression of IGF—1 to inhibit apoptosis and protect the cerebral tissue in ischemia. |
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