Inhibitory Effect Of Angiostatin On Retinal Neovascularization

Objective: To explore the effect of angiostatin to prevent and treat Retinalneovascularization ( RNV) , and it' s properbly way to inhibit NV. Methods :Rats were marked A . B . C group at random,A:IL-1a , B:AS, C:normal. There were 10 rats in each group. Injected IL-1 a to A and B group' s vitreous body, induced it to produce RNV,made Rat' s RNV model. Injected the same volumn NS to C group. After making the model, injected AS to B group' s vitreous body, Injected the same volumn NS to A and C, after 3 days draw out 3 rats from each group of A. B and C at random ,injected sodium fluorescein to trail vein and FFA; detected the nitric oxide and nitric oxide synthase' s concentration in the rest rat' s plasma at different period;extirpated eyeball, made pathology slice and immunity histochemistry dye0 Results:The model formed NV at the fifth day; there was NV in A group , later fluoscein leakage. rat' s eyeground vein branch was clear, no NV in B group . rat' s eyeground vein branch was clear in C group, there was no fluorescein leakage ;NO and NOS' s concentration in plasma of A group was the highest (30.69 + 3.62) ,(18.44 + 2.41) , and NO and NOS' s concentration in plasma of B group was lower than A group' s(21.47 + 3.23) ?(14.37 + 1.98), NO and NOS' s concentration in plasma of C group was lower than B group' s(22.68 + 4.55) , ( 11.86 + 1.67) .compared A and B ,C, the difference had remarkable meaning (P<0.05), compared B and C , the difference had no remarkable meaning(P>0.05);A group 7 eyes-140 sheets organize slice , found more endothelio cyte nuclei of new vessels extending from retina to vitreous, the everage number of endotheliocyte nuclei of new vessels extending from retina to vitreous in each slice was 24 (24.49+ 2.70) ,and the number of endotheliocyte nuclei of new vessels extendingfrom retina to vitreous was 8 (7.8 5+ 0.58) in B group, and C group-7 eyes-140 sheets organize slice , found the endotheliocyte nuclei of new vessels extending from retina to vitreous in fewness slice ,each sheet slice subter-1 (0.29+ 0.22) on everage ?Conclusions: Angiostatin has great inhibition effect to RNV 0 The properbly way is to inhibit RNV by preventing the integration of VEGF and receiptor ,induce NOS' s form decrease , and NO' s form decrease, thus AS play a part in inhibiting NV. AS maybe be a promising medicine in inhibitting and treating RNV.