Ginseng The Chuanxiong Injection Studies On Focal Cerebral Ischemia Efficacy And Mechanisms

Objective: To study the protective effect and the dose-dependence of Renshen Chuanxiong injection in treating focal cerebral ischemia in rats; and to explore the mechanism of preventing and curing the cerebral ischemia with the theory of Yi Qi Huo Xie of Traditional Chinese medicine.Methods: Model rats of cerebral ischemia, which were prepared by electionic solidified the middle cerebral artery, were randomly divided into seven groups: the sham control, the model control, the Ligustrazine-treated control, Nimodipine treated control, and three experimental groups of varying injection dose of Renshen Chuanxiong. The neurologic function, the area of necrosis, changes of tissue morphology, and ultrastrcuture of ischemia area, and the level of ET-1, t-P, PAI-1 in plasma , TNF-α, IL-1β,EAA in brain tissue and SOD, MDA in serum were evaluated.Results: 1. In all three experimental groups, injection of Renshen Chuanxiong reduced the neurological deficits and brain-infracted areas and increased the weight of the rat with local brain ischemia. The results were better than Nimodipine and Ligustrazine-treated group.2. In Renshen Chuanxiong injection groups, SOD activity significantly increased and MDA level considerably decreased, while in the model control group, the SOD activity decreased and the MDA level increased, the result was better than Nimodipine and Ligustrazine-treated group.3.Injection of Renshen Chuanxiong significantly decreased ET-1 level in plasma and the result of highest-doze group was better than Nimodipine and Ligustrazine treated group, but there was no significantly difference between low-doze group and Nimodipine and Ligustrazine treated group. The plasma ET-1 level of the model control group was higher than that of the sham control group.4.Injection of Renshen Chuanxiong significantly decreased TNF-α and IL-1β levels in brain tissue and the result was better than Nimodipine-treated group,but there was no difference between injection of Renshen Chuanxiong andLigustrazine-treated group. The TNF-α and IL-1 β levels of the model controlgroup were higher than those of the sham control group.5.Injection of Renshen Chuanxiong increased t-PA concention and activity and decreased PAI-1 concention. The t-PA concention and activity in the model control group was significantly lower than that of the sham control group, while the PAI-1 concention in the model control group was significantly higher than that of the sham control group.6.Injection of Renshen Chuanxiong decreased Glu level in brain tissue, there was no significantly difference between it and other comparative groups. The Glu level in the model control group was lower than that of the sham control group.7.Injection of Renshen Chuanxiong could lead to improvent of morphology and ultra-structure of rats with brain ischemia.Conclusions: Renshen Chuanxiong injection may protect from cerebral ischemia injury in rats, its synthetical propective effect is better than ligustrazine injection and nimodipine injection which is often applied in clinic. Through adjusting the diastolic factor of blood vessel, reducing free radicals harm,EAA in brain tissue, blancing the disorder of plasmin system and lowering antiinflammation action, Renshen Chuanxiong injection attain the effect of treating cerebral ischemia injury.