The Study Of Chinese Herbal Compound On The Follicle Control And Ovarian Expression Of Vegf And Igf-i Affect

ObjectiverTo study the effect of the GuiXiTang and ZichongKeLi medicatied by different ways upon the follicle development and the expression of VEGF and IGF-1 in the ovary of the rats.Method:early immature rats were divided into groups with treatments of GuiXiTang(No.l,No.2,No3) separately by the medication of clyster and oral perfus. Subcutaneous inbeded LNG was taken as control group.Ovarian index,ovarian follical ,corpus iuteurruovarian vessels,E2 and P in the blood ,uterus index and histomorphology of uterus were detected separately.Immature rats wre divided into groups with the application of GuiXiTang(No.l and No.3) and ZiChongKeLi separately by the medication of clyster and oral perfus. Subcutaneous inbeded LNG was taken as control group,while PMSG was also taken as the control group to ZiChongKeLi.AU the groups in the experiment of ZiChongKeLi were Subcutaneous inbeded with LNG except blank contol group.Ovarian VEGF and IGF- I were checked by immunohistochemical method.The others index were same with early immature rats besides cornification index.Results:The effect of GuiXiTang was similar with Subcutaneous inbeded LNG to early immature rats and Immature rats .They both can decrease corpus luteum, the ovarian vessels especially the big vessels, ovarian index, ,P in the blood, uterus index and glands fn uterus of early immature rats and Immature rats (P<0.05). GuiXiTang also can weaken the IOD,Mean IOD and Area of VEGF and IOD of IGF- I in their ovaries (P<0.05). The group LNG+ZiChongkeLi can incease the antal follicles,but it is not obvious defferent from LNG(P>0.05).Both groups of ZiChongKeLi can increase the glands and lining epithelium of uterus.To be mentioned,the group ZiChongKeli which was medicated by clyster can increase ovarian vessels, IOD of VEGF, IOD and Mean IOD of IGF-1 (P<0.05).It has a trend of ZiChongKeli to increase the preantral follicles.The group which was used LNG+PMSG manifested the increase of mature follicles, corpus luteum.ovarian index,P in blood,ovarian vessels especially small vesells,glands of uterus of mature rats(P<0.05).Conclusion:1.GuixiTang l(ErWuYou) has the similar action to decrease corpus luteum, the 10D,Mean IOD and Area of VEGF and IOD of IGF-1 with LNG.2.ZiChongkeLi has the effect to increase ovarian vessels especially the big vessels, IOD of VEGF, IOD and Mean IOD of IGF-1 ,glands of uterus and lining epithelium of uterus of the rats with inbeded LNG.3.One of the mechanism by which GuiXiTang and Zichongkeli influence the ovary is to regulate the ovarian microcirculation and the expression of VEGF and IGF- I in the ovary.