| The important pathophysiology process of left ventricular remodeling after AMI including myocyte hypertrophy and increase of collagen etc, which are main reason of left ventricular dysfunction and heart failure. A great deal of attention has been focused on the inhibition and attenuation of this process. It has been proved by some studies that some medicines, such as ACEI, ATI can prevent or reduce left ventricular remodeling. As yet we have not found any reports about treating myocardial fibrosis with chinese tranditional herbal. It has been reported that herbal compound 861 can inhibit and reverse liver fibrosis after hepatitis, furthermore it has been proved to be effective in clinic. It possible that herbal compound 861 also has reversal effect on myocardial fibrosis, because liver fibrosis and myocardial fibrosis have common pathosiological features. The objective of this experiment is to investigate the effects of herbal compound 861 on myocardial fibrosis after acute myocardial infaction in rat.Materials and Methods1. Materials:1. 1 Experimental animals: 60 male SD rats were used, weighting about 250 g.1. 2 Medicine in experiment: herbal compound 861 made in medicine center of Beijing Friendship Hospital.2. The establishment of AMI model, grouping and treatment: 2. 1 Estalishment of AMI model; AMI model was produced in rats by ligation of LAD. The success of ligation was proved by EGG with ST segment elevation on v1 ~ v6 lead.2.2 Grouping; All of the models were divided into 6 groups at random with ten in every group. That is; group M14(without any treatments , killed at 14 days after infarction) ; group M56 ( without any treatments, killed at 56 days after infarction) ; group T14(treated with herbal compound 861 for 14 days before killed) ; group T56 (treated with herbal compound 861 for 56 days before killed) ; group S14(sham operation, killed at 14 days after surgery ) ; group S56 ( sham operation , killed at 56 days after surgery).2. 3 Treatment: 24 hours after operation, the herbal compound 861 (10ml/kg/day) was given for 14 days (in group T14) or 56 days (in group T56) . To weight body mass of models once 2 weeks and adjust dosage according to alteration of body mass. All rats were given standard rat chow and water.3. Assay indexes:3. 1 BW, HW, LVW of rats were measured when the rats were killed.3.2 Collagen contents; The collagen contents of left ventricle were determined by the hydroxyproline assay. The hydroxyproline contents were measured with the chioramine T method.3.3 Histopathologic observation; The heart was quickly removed and a 2 to 3 mm coronal section, taken from the equator of the heart was fixed in 10% formalin and embedded in paraffin. Paraffin sections (4um) were stained with masson, observed with light microscope.4. Statistics :All the data were expressed as x ?s , and analysis of t test were taken for testing. The level of significance was taken at p <0. 05.Results1 . BW of all the rats existed non - significant difference ( p > 0.05). There were not significant differences in HW, LVW, HW/ BW, LVW/BW between group M14 and group S14, but these indexes in group M^ were higher than those in group S56 (p < 0. 01). HW, LVW, HW/BW, LVW/BW in T groups were lower at 14 and 56 day than those in M groups at corresponding time (p <0. 01).2. Collagen contents of T groups were lower at 14 and 56 day than those of M groups( p <0. 01) , but were still higher than those of S groups(p<0.01).3. Histopathologic observation; A great deal of collagen fibers deposit in infarct zone and non - infarct zone. Collagen deposition declined after treatment of herbal compound 861.DiscussionThe acute and long - term change in ventricular topography ( ventricular remodeling) after AMI are important process affecting ventricular function and survival. Ventricular remodeling include dilatation and thinning of the area of infarction and myocyte hypertrophy and interstitial fibrosis in non - infarct area. The collagen increase and myo... |
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