Nf-¦Êb In Cerebral Ischemia And Reperfusion Role Of Local Inflammatory Reaction In The Brain Tissue

The effect of NF-κB on inflammatory reaction of brain tissue after cerebral ischemic reperfusion.Along with increase of incidence of rate cerebrovasu1ar disease and development of early diagnostic technique of cerebral infarction early thrombolysis treatment has been widesperad application in clinical. Continuous lesion of brain tissue after cerebral ischemic reperfusion has become hot point of study .A great deal of domestic and overseas studies indicated that ultra focal inflammatory reaction of brain tissue after cerebral ischemic reperfusion was one of main reasons causing in jury. It has been proved that monocyte/macrophage infiltration in over inflammatory reaction concerned with expression of cell chemokine MCP-l.The specific mechanism of M C P- 1 expression induced by inflammatory cytokine was still unknown today. The aim of this study was to investigate the expression of NF-κB and M C P- 1 in brain tissue after cerebral ischemic reperfusion at varying intervals, the influence of MCP-1 expression after antioxidant N-acetylcy -steine (N A C) inhibited, the mechanism of MCP- 1 expression increase after cerebral ischemic reperfusion .This research would provide theory background for ischemic cerebrovascu1ar disease.We used animals model of Zea-Longa et al. The modelof sham-operative group except no occlusion with thread was identical with that of experimental group. All animals were killed at given time, and then perfused. The brains were removed and cut into 11-U m thick successive coronal were observed by HE dye, and the expression of MCP-1 and NF- K B p65 was determined with the method of immunohistochemisty .Results of HE dye after cerebral alchemic reperfusion showed: Cortex was infiltrated by neutrophil; Cells of corpus striatum and cortex of parietal lobe were stained light; the number off cells obviously reduced; the feature of cells necrosis, such as cells swelling, nuclear fragmentation and lysis et al, would be observed. Vascular endothelial cells pyknosis, chromatin concintration and edge accumulation was seen under high poly-microscope . Two hemispheres of sham -operative group didn' t appear above -mentioned changes. Inflammatory cells infiltration evidently relieved after antioxidant N A C treatment prior to ischemic and reperfusion.Results of immunohistochemistry under microscope are following: MCP-1 positive stain being brown particles was chiefly located cells plasm. There was no positive cell in brain tissue of normal control group. There were a few light stain MCP-1 positive cells in right cerebral ependyma at 2h ischemic. There were scattered light stain positive cells in hippocampus at 4h ischemic. There was expression ofMCP-1 at 12h and later ischemic, while it reached peak at 24-48H.Dark stain cells plasm spread in some parts. Immunohistochemistry results showed:(1) There was no expression of MCP-l and NF-κB P65 in brain tissue of sham -operative group in rat .(2) Expression of brain tissue. MCP-l in ischemic reperfusion group markedly increased. There was little expression at 15min.The expression began to increase at 2h. . It reached the peak at 24-48h and gradually decreased at 72h.(3) Expression of brain tissue N F - K B P65 in ischemic reperfusion increased. It reached the peak at 15-30min.There was expression at latter different time.(4) Expression of brain tissue N F-K B p65 and MCP-l after N A C treatment at 1 h prior to ischemic obviously decreased at different time. It was significant difference as compared with control group (p< 0.05)(5) Expression of brain tissue N F-K B p65 and MCP-l after N A C treatment at 1 h after to ischemia obviously decreased at different time. It was significant different as compared with control group(p<0.05).(6) There was no obviously difference in expression of NF- K: B p65 and MCP-l between Ih prior to ischemia and Ih after ischemia of NAC treatment.Conclusions: It increased that expression of NF-K B P65 and MCP-1 after cerebral ischemic reperfusion. Antioxi...