Laryngeal Cancer Of Mage-1 Gene Promoter B'b District To The Methylation Status Of Research

Objective: To study the methylation state within MAGE-1 B'B promoter in laryngeal carcinoma and the assocication between demethylation and pathological differentiation, the association between demethylation and clinical stage. Methods: Using methylation-sensitive restriction analysis followed by polymerase chain reaction(PCR),we studied 34 specimens that were obtained from surgical samples(including 27 laryngeal carcinoma and 7 benign or normal tissues). Each specimen was stored at -80℃ until used. Disease stages were determined using the TNM classification according to UICC criteria. Results: An demethylation state was identified in DNA from laryngeal carcinoma specimens. The demethylation rate is 29.6%(8/27). In contrast, no demethylation state was identified in DNA from benign or normal tissues. The differences were significant statistically. In our study, the demethylation in poorly, moderately, and well differentiated squamous-cell carcinoma were detected at frequencies of 62.5,25 and 9.1%, respectively. The differences were significant statistically (P<0.05). The demethylation rate in early, late tumor stage was 40 and 16.7%, respectively. But the differences were not significant statistically. Conclusion: The demethylation in MAGE-1 B'B promoter is anomalous in laryngeal carcinoma, and maybe is related to the expression of MAGE-1 gene in laryngeal carcinoma. The demethylation rate is related to the pathological differentiation, and is not related to disease stages.