Ferulate And Captopril On The Isolated Rat Heart Pharmacological Preconditioning Protective Effect And Mechanism Of Experimental Research

Objective: To study the protective effect of pharmacological precondition induced by sedium ferulate (SF) and captopril (CP) on isolated rat hearts and machanisms. Methods: Isolated SD rat hearts were Langdorff perfuted(perfusive solution: Ringer-locke) and divided into 6 groups at random: Normal group (hearts were perfused by oxygenated perfusate for 100 minutes); IschemialReperfusion group (Group I/R, hearts were subjected to 30-minute global ischemia and 30-minute reperfusion); Ischemic precondition group (Group IP, hearts were preconditioned by 5-minute global ischemia/5-minute reperfusion for three times before they were subjected to 30-minute gfobal ischemia/30-minute reperfusion); Group SF (hearts were perfused previously with oxygenated perfusate administered with 0.21 mmol/L SF and subjected to 30-minute global ischemial30-minute reperfusion); Group CP (hearts were perfused previously with oxygenated 3 perfusate administered with 0.01 mmol/L CP for 15 minutes and subjected to 30-minute global ischemial3 0-minute reperfus ion); Group SF+CP (hearts were perfused previously with oxygenated perfusate administered with 0.2lmmolIL SF and 0.Olmmol/L CP for 15 minutes and subjected to 30-minute global ischemial30-minute reperfusion).Electrocardiography of the isolated hearts, transmission eletronmicroscopy of myocardium, detennination of myocardial MDA, activity of GSH-PX and SOD, ATPase and Ca2~ contents were performed and analyzed statistically. Results: Compared with f/R group, 0.21 mmol/L SF or 0.01 mmol/L CP preconditioning increased coronary flow, improved heart function; enhanced activity of superoxide dismutase (SOD) and glutathion peroxidase (GS H-PX), reduced malondialdehyde (MDA) content, alleviated calcium overload in cardiac myocytes, reduced the incidence and severity of ventricular arrhythmia; furthered activity of Na~桲~ ATPase, Mg2~桝TPase and Ca> ATPase in myocardium. But the combined use of SF and CP didn抰 show any significant promotion in protective effect on myocardium . Transmission el ectronimcroscopy showed turgescent myofibrils of cardiac myocyte in group hR with indistinct myofilaments. The mitochondria swelled; its matrix was pale; the mitochondrial crista were shortened, lessened and irregularly 4 arranged; many of them were vacuolized or fractured with granular appearance. The sarcoplasmic reticulum showed vacuolar enlargement (Fig. 1). The ultrastructure of cardiac myocytes in groups SF, CP and SF+CP showed no significant improvements. There was no thinning of the cardiac muscle fibers. The myofilaments arranged regularly and distinctly. The mitochondria had nomal size and complete structure (Fig.2,3,4). Conclusion: 1 .Both SF and CP have protective effect of pharmacological precondition on the isolated rat heart. 2.The rnachanism of protective effect of pharmacological precondition of the SF and CP may be similar. 3. The combined use of SF and CP doesn抰 result in significant promotion, so it抯 not necessarily advocated.