Magnesium Sulfate, Glutamine Preventive Role Of Bacterial Translocation In Rats With Acute Pancreatitis

Under the condition of acute pancreatitis(AP),in order toinvestigate the effect of Magnesium Sulfate (MS)-induced diarrhea andthe effect of this therapy in combination with enteral provision ofGlutamine(GLN) on pancreatic infection,intestinal mucosa andintestinal bacterial transloction and to c1ariI~,?the mechanism of thesetreatments, firstlv,6Wister rats were used as a sham group to validatethe correctness of the operation method Then 15 1 Wister rats weredivided into 3 groups(every group were divided into 5 subgroupsaccording to the sacrifice time): l,control group (n1 O),APmodelwithout treatment ;2,MS group (n=50)AP model without treatment; 2,MS group (n=50) received enteral Magnesium Sulfate after inductionof AP; IGLN+MS group (n=5 1) receival entered L-Glutaminc (GLN)and Magnesium Sulfate after induction of AP.All rats in each group were sacrificed at 24,48,72,96.144 hr afteroperation respectively. Bacterial cultures were obtained from pancreas.spleen, liver, mesenteric lymph nodes (MLN), portal blood and thecontents in cecum. All the procedures were performed under sterileconditions. Morphohological changes ofjujunal mucosa were observedunder light microscope, villi height and mucosa Lhickness weremeasured by microscope automatic image analyzer.Result shows that:(1)The incidence of positive MLN cultures; the positive rate of4MEN cultures iii control group was g900 1000010000 at 72. 96 144hrrespectively and 330o . 44%. 44% in MS groups . which weresi~niticantk lo~~er than control 2roup( p (1.05 .The positive rate was33%. S3~抩. 44% in MS桮LN 2roup . also si~niticant1v Io~\er thancoiitr拻d gI扥U1~ p ~ )?2 ) pOsItl\ e rate of pancreu~ ctthure>. the positive rate ~ as S7~ o.7 1 搤 at 96. 1 JJhr in control group. I he positive rate in (iLN ?MS~roup \~U5 11%. 2200 at 96. lJJhr v~hich ~~ere signitic ant1~ lo\~er thancontrol ~rotip (p 0.05).(3) There 憕as no statisticall?si~niticaiit ditl鑢eiice of bacterialculture positive rate in liver and portal blood between the groups.4 Cecal colony counts:the cecal E.coli amount at 72.96.144hr inboth MS and MSi-GLN group x~as si~nificant1v decreased incoplparison to control. In addition. the cecal E.coli amount at 72.96.1441w in MS-~-GLN group was si2niticantlv lower thaii that in MSgroupMorpholmlo~ical changes of je~unal mucosa: there ~~ereerosioii and necr?sis ot mu~osa and sheddiiw of vi lii in the coil trol andMS group.Hn~vever. there was hardly any mucosal lesion in MS桮LN2roup. both nih height and mucosa thickness were significantly higherthan control and MS group.T1i~re results suggest that: inhibition of intestinal peristalsis wasthe key process leading to pancreatic infection due to intestinalbacterial translocation after acute pancreatitis. Magnesiuni Sulfate canpromote intestinal peristalsis. reduce intestinal bacteria and toxins(ilutarnine can help to keep the mucosal integrity and barrier functionul the intestuie. decrease the bacteria positive rate in pancreas.Combined application ot MS and (ii N has prominent effect onprevention ul?iiite~iiiial bacterial translocation and decrease otpancreatic. in tcCtio~i alter ac tite p~i;lcveat ills.