Marine Fungal Secondary Metabolites

Bioassay Guided by cytotoxic activity in vitro such as killing or inhibiting the growth of Shrimp, four strains of fungi have been isolated from anemona growing in Qingdao beach, China. They are identified as Penicillium thomii A, Aspergillusflavipes B, Fusarium sp. C and Rhizopus sp. D. From the fermentation broth of the strians A and B,Twenty five compounds were isolated. Structures of these compounds were elucidated mainly by using spectroscopic methods, On the basis of physical and chemical properties and spectroscopic method(扝NMR~ ?CNMR~ DEPT~. EI-MS~. FAB-MS~. 慔-扝 COSY. ?C-扝 COSY. HMQC~ HMBC-. NOESY~ 1DTOCOSY), 21 compounds were elucidated as 4-hydroxy benzeneethanol (1 ,Jae8), N-[2-cis(4梙ydroxyphenyl)ethenyl] formamide(2,Jae9), N-[2-trans (4-hydroxyphenyl)ethenyl]formamide(3,Jae5), 1 ,2-Benzendicarboxylic acid-3-ttN~i ~ABSTRACTrwn,.rflrryrr/aSWrflSyflSr/rrrflw/flW/flrfl,WS>flflzfl/Wfl/Srrrfl/Sflbis [2-methylpropyl]ester(4,Jae 1), p-Methylbenzolic acid(5,Jae7), 1 -Q-hexa decanoyl?--O-(9-octadecenoyl)?桹-(9, I 2-octadecadienoyl)glycerol (6,Jtf 11), 5 a ,8 a -epidioxy-24 ~ -methylcholesta-6,22-dien-3 13-ol(7,Jaal), 1,6,8trihydroxyl-3-methyl-9, 1 0-Anthracenedione(8,Jaa3), (1 O,Jaa6), Ergosta-5 ,7,22-trien-3 ~-o1(11 ,Jba2), (3 J3,4~,22 j3)olean- 1 2-ene-3 ,22,23 -triol(12,Jba3), 1-05Z,8Z-Octadecadienoylglycerol(13,Jba4), (14,Jba5), 1 ,2,3,4-Butanetetrol(15, Jba6), daucosterol(16,Jba8),uracil(17,Jba 10), 5梙ydroxy-2梙ydroxymethyl411-pyran-4-one(18,Jbal ?, (4E,8E)? 桹-~3-D-g1ucopyranosyl)?-(2-hydroxy heptadecylamido)-3 -hydroxy-9-methyl-4, 8-nonadecadiene(1 9,Jba 11-2), (4E, 8E)? ?-(f3-D-glucopyranosyl)?-(2-hydroxy-(E)-3 -heptadecenoylamido)-3 hydroxy-9-methyl-4,8-nonadecadiene(20,Jba li-i), (4E, 8E)?1 ?-(~3-D-ga1acto pyranosyl)?-(2-hydroxyheptadecylamido)-3 -hydroxy-9-methyl-4, 8-nonadeca diene(2 1 ,Jbal 2-2), (4E, 8E)? ?-(~-D-ga1actopyranosy1)?-(2-hydroxy-(E)-3 heptadecenoylamido)-3 -hydroxy-9-methyl-4,8-nonadecadiene(22,Jba 12-1), respectively. Among them compounds 10, 14, 19, 20, 21, 22 are identified as new compounds.