Four Gentlemen Decoction Together, Points A Comparative Study Of The Chemical Composition Of The Decoction

The granule of one ingredient is a try of reformation of the traditional. decoction. In order to approach the feasibility of the use in medicinal formula of the granule of one ingredient,The Decoctlon of Four Noble Ingredients was chosen as the object of study. Decoction of Four Noble Ingredients sterms from song dynasty "Benevolent Prescriptions from the Pharmaceutical Bureau of the Tai Ping Period" composed of Radix Codonopsis Pilosulae, Poria, Rhizoma Atractylodis Macroce- phalae, Radix Glycyrrhizae. It possesses the effectiveness of the replenishing qi and invigorating middle- jiao, invigorating the spleen and nourishing the stomach. The chemical compositions have been defined, including Flavonoids, Saportin, Polysaccharide and Glycyrrhizin.The feasibility ofanalytical method ofthese chemical compositionswas studied. The resultsof quantitative analysis indicate that the content of these chemical compositions in the mixed decoction is hlgher.The qualitative analysis of different decoction was studied.The collection of illustrative plates is the same. Therefore, the relevant department must pay attention to the technological preparation of the granule of one ingredient and the internal quality standardization. 1. Assay for extract According to the Pharmacopoeia of the People's Republic of China, the content of extract was determined. 2.Assay for Flavonoids Separating samples on a polyamide column , the content of Flavonids was determined by UV at A z4llnm The linear range of calibration curve is 4000 g(r0.9998).The average recovery was 89.35% with the relative standard deviation 2.74%(n=5).The method is simpie,sensitive and reIl iahle. 2.Assay for Polysaccharide UV mcthod was used with phenol ulfuric acid as developing reagent( A 490nm), the linear range was tO. 12 50. ig(r0.9992).The average recovery was 92. 39% with the relative standard deviation 2.05%(n4).The effect of centrifugation and filtering have been compared.The recovery of filtering is lower than centrifugation. 3. Assay for Saponin Samples were purified with a column of nacroporous resin and washed with 300m1 distilled water, lOOml 10% ethanol,then eluted with lOOml 95% ethanol.The content of Saponin was determined by IJV at A 272nm.The linear range was 8.4 2 it g ( r0.9998 ).The average recovery was 97. 54%(RSD=3.92%, n5).The method is stable. 4. Assay for Glycyrrhizin A specific and sensitive HPLC method for the assay of Glycyrrhizin was developed..lJsing methyl alcohol( 0.2M sodium acetate:2% acetic acid(l:2) ) (63:37) as floating phase,floating velocity l.Oml/min, A 250nm,the linear range was 1-5 it g(rO.9998). The average recovery was 98. 19%(RSD1.25%).The method is accurate,simple and reproducible.