Contrast Observation, The Guinea Pigs In Different Parts Of The Extrahepatic Biliary Ischemia Reperfusion Injury

Background A large amount of studies have demonstrated that extrahepatic bile ductal tissues, just as hepatic tissues, displayed injury after hepatic arterial ischemia. The tissues of extrahepatic bile duct ischemia may cause bile strictures and leaks, so the study of extrahepatic bile ducts ischemiaeperfusion injury is apprectiated nowadays. However, no report on the comparative study of ischemiaeperfusion injury to different parts of extrahepatic bile ducts have been seen. In our experiment, the guinea pig was used as a model for studying on ischemiaeperfusion injury to the tissues of the common hepatic duct, the common bile duct and the gallbladder. Methods The adult guinea pigs were divided into three groups of 12 animals each. Sham operation group (group 8): sham operation and no blocking hepatic portal blood flow; Ischemia group (group I): the hepatic artery, the hepatic portal vein and the common bile duct were clamped above duoderum for 30 minutes; Ischemia梤eperfusion group (group IR): after manipulating as group I, extrahepatic bile ducts blood flow were resumed for 60 minutes. All animals including group 8, group I and group JR were sectioned the common hepatic duct, the common bile duct and the gallbladder to investigate superoxide dismutase (SOD). In every part of these bile ducts, a small mass of tissues was sectioned for electronicroscope sample, then alterations of organelles were observed, mumerical density(Nv) and mean volume (V) of mitochondria of epithelial cells were counted by an image analysis system. Results 1. Alteration of morphology: In group 8, cristae of mitochondria were clear and microvilli in the margin of epithelial cells were rich; In group I, the mitochondria were swollen, some cristae were obscure and some microvilli exfoliated; in group JR, the mitochendria were more severely swollen, some cristae were obscure 6r small, microvilli were sparse. 2. The feature and difference of bile duct ischemia? reperfusion ingury in different groups animals (see table 1) TABlE 1 Mitochondrial V, Nv and tissular SOD of extrahepatic bile duets in animals in of different groups (knin3n Bile Duct by group IR + Values of mean increase, P<0.05; Values of mean increase, P<0.0l;梀alues of mean decrease, PK0. 05; Values of mean decrease, P(O. 05; alues of mean no marked difference, P>0. 05. 3. The feature and difference of extrahepatic bile ducs ischemiaeperfus ion injury in different parts. (See table 2) TABLE 2 Mitoclidondrial V, Nv and tissular SOD of different parts of extrahepatic bile ducts of mean decrease, P(0.05;?Values of mean no marked difference, P>O.05. Conclusion 1. After 30 minutes of ischemia and 60 minutes of reperfusion, all extrahepatic bile ducts in guinea pig displayed injury and the injury of reperfusion was more severe than that of ischemia. 2. The injury to different parts of. extrahepcetic bile ducts in guinea pigs were evidently different on the same condition: after 30 minutes of ischemia, the injury of common hepatic duct and gallbladder were more severe than that of the common bile duct; After 60 minutes of reperfusion, the most severe injury among the extrahepatic bile ducts in guinea pig was gallbladder, the second severe was common hepatic duct, the last severe was comm...