Adiponectin Inflammatory Mediators In Septic Rats The Expression Of Experimental Research, The Impact And Prognosis

Objective To investigate changes in adiponectin expression and to observe the relationship between adiponectin and tumor necrosis factor-α(TNF-α) in septic rats.Methods Sepsis model of rats was replicated by intravenous administration of LPS into the tail vein. Forty-eight male Wistar rats were randomly divided into two groups, respectively the normal control group (n=24), and the LPS group (n=24). At 4 hours and 24 hours after administration of NS or LPS, rats were sacrificed by heart phlebotomy. Epididymal adipose tissues were sampled. The expression of adiponectin mRNA and TNF-αmRNA in the adipose tissue were detected by Quantitative real-time reverse transcription-polymerase chain reaction (Real-time PCR) with GAPDH as an internal standard. The levels of adiponectin and tumor necrosis factor-α(TNF-α) in plasma were determined by enzyme linked immunoadsorbent assay (ELISA).Resluts Adiponectin mRNA and TNF-αmRNA were slightly expressed in adipose tissue of rats in normal control group. The expressions of TNF-αmRNA and levels of TNF-αin plasma were increased at 24 hours after administration of LPS, peaking first at 4 hours .The adiponectin mRNA was decreased at 24 hours. The level of adiponectin was decreased at 4 hours and 24 hours.Conclusion These data indicate that there is a marked decline in the adiponectin level both in plasma and epididymal adipose tissue, which corresponds to an increase of the levels of TNF-α. Adiponectin plays a role in the uncontrolled inflammatory reaction.Objective To investigate the effect of adiponectin on early and late cytokine expression in lipopolysaccharide(LPS) induced septic rats.Methods Ninety-six male Wistar rats were randomly divided into 4 groups: control group (C group, n=24), model group(LPS group, n=24),early adiponectin treatment group(APN+LPS group, n=24), late adiponectin treatment group(LPS+APN group, n=24). At 4, 24, 48 and 72 hours after administration of NS or LPS six rats were randomly sacrificed in each group, serum samples and the lung tissue samples were collected. The levels of TNF-αand interleukin-6(IL-6) in plasma were determined by ELISA. Lung tissues were sampled. The expressions of high mobility group box 1 mRNA in the lung tissues were detected by Quantitative real-time reverse transcription-polymerase chain reaction (Real-time PCR) with GAPDH as an internal standard. The survival curves in 4 groups were drawn.Resluts The levels of TNF-αand IL-6 in plasma in LPS group were higher than those in C group(P<0.05), last for 24 hours. The TNF-αand IL-6 levels both in APN+LPS group and LPS+APN group were reduced as compared with LPS group (P<0.05). The HMGB1 mRNA expression level in lung tissue was measurable at 24 hours in LPS group (P<0.05), and maintained a high level at 48 hours after administration of LPS. The HMGB1 mRNA expression levels in both of APN+LPS group and LPS+APN group were decreased as compared with LPS group (P<0.05). The survival rate of rats both in APN+LPS group and LPS+APN group were higher than LPS group (P<0.05).Conclusion Adiponectin can raise the survival rate of septic rats significantly, it also can inhibit the early and late cytokines expression in LPS induced septic rats.Objective To investigate the effects of rosiglitazone on the outcome in rats with sepsis.Methods In a sepsis model by intravenous injection of LPS, forty-eight male Wistar rats were randomly divided into 4 groups: control group(n=12), model group(n=12), early rosiglitazone treatment group(n=12) and the therapeutic treatment group(n=12). At 24 and 48 hours after administration of LPS or NS, rats were sacrificed. Blood samples were collected to determine the serum HMGB1 and the serum concentration of adiponectin. Organ functional parameters were still determined. The survival curves in 4 groups were drawed.Results The serum HMGB1 levels were negative correlate with the changes of the serum adiponectin levels (r =-0.889). Compared with control group, the serum HMGB1 levels increased in rosiglitazone treatment group and sepsis group (P<0.05), although the results in rosiglitazone treatment group markedly decreased than that in sepsis model group (P<0.01). At 24 hours after administration of LPS, the serum adiponectin levels decreased in rosiglitazone treatment group and sepsis group compared to the control group (P<0.05), although the results in rosiglitazone treatment group markedly increased than that in sepsis model group (P<0.01). No significant differences in serum adiponectin levels were noted at 48 hours after administration of LPS among the 4 groups. Compared with sepsis group, the serum creatine kinase(CK), alanine aminotransferase(ALT), aspartate aminotransferase (AST), creatinine(Cr) and blood urea nitrogen(BUN) levels were markedly decreased in rosiglitazone treatment group(P<0.01), and PaO₂ were also significantly elevated(P<0.01). The survival rate of rats in both early rosiglitazone treatment group and the therapeutic treatment group were higher than that in model group (P<0.01).Conclusion Rosiglitazone can raise the survival rate of septic rats significantly, it also can inhibit HMGB1 expression, induce the production of adiponectin and prevent the development of multiple organ dysfunction secondary to sepsis in rats.