Heat Blood Circulation Compatibility In Addition To Mice Coronary Atherosclerosis In Diabetic Apoe Gene Knock

BACKGROUND:Diabetes mellitus is a disease of chronic metabolic disorder, and it has become the third largest serious threat to human health of chronic non-communicable diseases. In recent years, the incidence of diabetes mellitus is increasing. Compared with the non-diabetic population, atherosclerotic disease rates higher, onset ages lighter, progress faster, and more viscera simultaneously involved in the diabetic population. In addition, heart cerebrovascular disease triggered by atherosclerosis is one of the main causes of death and disability in the diabetic population. Large numbers of studies confirm, atherosclerosis exists in the early stage of diabetes, and they are closely related. Now the low-grade inflammation is considered to be one of the important factors causing and promoting atherosclerotic disease with diabetes. According to the TCM theory, phlegm retention\blood stasis is regarded as the main pathological product of low-grade inflammation, phlegm and blood stasis mutual knot is the basic pathologic characteristics of low-grade inflammation, so we choose to study the influence of qingrehuoxue Chinese medicines on the inflammation of Apo·E knockout mice with diabetes mellitus and coronary atherosclerosis, thus providing scientific basis and new ideas for TCM in the treatment of coronary atherosclerotic disease with diabetes.AIM:To investigate the inflammatory reaction changes on coronary artery of Apo E deficient mice with diabetes mellitus by treating with QingReHuoXue method, providing the scientific basis and new ideas for Chinese medicine in the treatment of coronary atherosclerotic disease with diabetes.METHODS:40 male Apo E deficient mice were given injected with 60 mg/(kg·d) streptozotocin for 5 days to induce diabetes mellitus with fasting glucose>10mmol/L. The Apo E deficient mice with diabetes were classified into the QingReHuoXue group (n=20) and control group(n=20) randomly. At the end of the study we measured the blood glucose and cholesterol level,monocyte/ macrophage-2(MOMA-2),coronary-artery collagen content and evaluated the Main Coronary Artery Stenosis\Occlusion and inflammatory infiltration surrounding the coronary artery of the two groups. We also measured the expression of ICAM-1,MCP-1 and HO-1 by Real-time PCR.RESULTS:By the end of experiment, the MOMA-2 content(QingReHuoXue group: 29.09%±3.12%, control group:58.06%±5.49%), coronary-artery collagen content (QingReHuoXue group:5.23%±0.63%, control group:13.77%±1.05%), main Coronary Artery Stenosis (QingReHuoXue group:37.3%±3.87%, control group:43.1%±4.99%), main Coronary Artery occlusion(QingReHuoXue group:17.2%±1.00%, control group:29.3 ±0.73%) and Inflammatory cells density surrounding coronary artery(QingReHuoXue group: 17.9%±1.97%, control group:26.1%±2.77%) were significantly reduced in the QingReHuoXue group comparing with the control group (P<0.01).There are also obvious decrease of the fasting blood-glucose level after 15 weeks, serum advanced glycation end products in the QingReHuoXue group(P<0.05), The expression of ICAM-1 and MCP-1 were significantly reduced (P<0.05), the expression of HO-1 was significantly higher than that of the control groups (P<0.01).CONCLUSION:The QingReHuoXue method can inhibit the inflammation reaction of coronary artery intima and adventitia, and the gene expression of inflammation factors in the mice with diabetes mellitus and atherosclerosis, meanwhile lessen the main coronary artery stenosis and occlusion, slow down the progression of coronary artery atherosclerosis.