| Research purpose:In order to know Rhizoma Curculiginis in the body of pharmacokinetics characteristics, and observe medication after different time saliva and blood drug concentration in the dynamics of change, and get the saliva and blood pharmacokinetic parameters of the correlation between the saliva, so that through the non-invasive diagnosis of drug concentrations to detect drug exploitation degree, to better service to clinical medication or sports practice.Research methods:Will Rhizoma Curculiginis picked clean impurities, weighing medicinal 1250g, join 10.75 L soaking water, gas burner heating, stay 2h boiling water, after fire moves to continue decoction 0.5 h, which in turn with 4,6 and 8 layer gauze filtering, again to add liquid 5L water, boiling water, soft fire archives after decoction 0.5 h, filtering, with the filtrate drawees, using drying will water extraction liquid enrichment to 0.42 g/ml crude; Subjects after eating bland dinner before test fast 12 h overnight, in the morning hollow oral fairy spear water extractings from solution. Participants in medication after 0.5,1.0,1.5,2.0,2.5,3.0,4.0,6.0, 8.0, and 10.0 h take some blood from the forearm vein collected saliva and 2 ml 2ml. Blood heparin sodium anticoagulation, centrifugal separation plasma; Saliva collect completely before mouthwash, centrifugal take supernatant fluid, in-84℃cryopreservation. Determination of plasma and saliva room temperature will stay melts, the place to protein processing junior liquid chromatography, use external standard method to calculate the grape polyphenols containing moss black respectively, and rkatsiteli DAS2.0 software concentration of each participant give pharmacokinetic parameters, find out including drug concentration and pharmacokinetics of correlation between the parameters.Experimental results1. The subjects of different gender in taking rhizoma curculiginis water extract ingredients orcinol glucoside enter the blood in saliva and blood the concentration ratio (S/P) range between 0.257～0.443 in 0.350+0.038, an average of about; Plasma pharmacokinetic parameters no significant differences between men and women; Saliva pharmacokinetic parameters between the men and women also no significant difference (P< 0.05)2. Within 0.5～10h after taking the drug, each time point saliva, plasma containing the orcinol glucoside concentration correlation coefficients of 0.723,0.676 respectively r 0.613,0.746 0.930, and 0.624,0.660,0.798,0.921,0.776, (P< 0.05), an average of 0.747.3. Orcinol glucoside reached peak time in saliva with plasma is highly synchronicities, through the saliva and plasma calculated two methods t1/2, Ke, Ka, no obvious difference tl/2Ka (P> 0.05)4. Rhizoma curculiginis into blood composition orcinol glucoside in the body of t1/2 of 2～3h, between 1～4h for rapid disposal drugs. The experimental conclusions1. Participants were taking rhizoma curculiginis water extraction after fluid, gender, age, enter the blood of rhizoma curculiginis effective ingredients orcinol glucoside in saliva and plasma of concentration ratio (S/P) no influence; Sex of plasma respectively pharmacokinetic parameters, saliva pharmacokinetic parameters have no effects.2. Orcinol glucoside the concentration saliva and in plasma concentration exists in the height of dynamic parallel with saliva, can replace correlation as the test to determine the plasma before taking rhizoma curculiginis water extraction after fluid its orcinol glucoside concentration, and available orcinol glucoside by DAS2.0 software determination of the saliva pharmacokinetic parameters can replace plasma pharmacokinetic parameters to guide clinical medication or athletes, we can promote saliva drug determination of its pharmacokinetics parameters determination which generalized without traumatic in the sport of the bioavailability. |
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