Massage Intervention Study On The Expression Of Peripheral Nerve Injury In Rat Spinal Dorsal Horn In The Ntfs

【Objective】Based on the good curative effect of traditional chinese massage therapy, the repairing function of endogenous neurotrophic factors to the damage nerves, take experiments to verified massage could shorted the repairing period of the damaged nerve by promoting endogenous neurotrophic factors expression and the cycle pathways of neurons nerve-muscle for the further explore established massage effect mechanism and function approach lay the foundation.【Methods】The study take SD rats as experimental animals, the model of sciatic nerve injured by clamping, randomly divided into normal group, model group, model control group, massage therapy group, using the massage simulator to intervene, observed behavioral indicators of change: Rivlin' oblique plate test, heat pain threshold, Pain sensitive scores; Morphological changes such as the muscle cell diameter, the shape of spinal cord neurons and so on; the expression of nerve growth factor changes by SABC immunohistochemical【Result】Rivlin' oblique plate test, gastrocnemius wet weight, gastrocnemius muscle recovery rate, massage therapy group were better than in model control group (p<0.05);The paw withdrawal thermal latency, Pain sensitive scores, massage therapy group were less than model group (p<0.05);The diameter of muscle cells observed by light microscopy, massage therapy group were higher than model group (p<0.05); the number of surviving neurons in the spinal cord, massage therapy group were larger than the model control group;Immunohistochemical staining, compared with model group,massage therapy group can effectively promote the body NGF, bFGF secretion (p <0.05), has some effect in promoting the secretion of BDNF, but the difference between message group and model group was not significant; role in promoting the expression of CNTF is not obvious, need a further study. 【Conclusions】There is a genuine effect of the treatment of peripheral nerve injury by massage, massage therapy can effectively promote the endogenous neurotrophic factors expression such as NGF,bFGF,affect the expression of BDNF, CNTF, the massage thereby protecting neurons in peripheral nerves, and promote repairing and regeneration of the injured nerves; stimulate regeneration and functional recovery of the target organ (Muscle) administrated by nerve; accelerated neuronal nerve-target organ recovery pathway to improve the coordination of damaged nerve regeneration comprehensive.