Reduce Focal Cerebral Ischemia In The Mechanism The. Notoginsenoside Role In Not Ischemic Brain Regions

Objective:In Chinese medicine under the guidance of the overall concept, through animal experiments to observe the PNS by acting on the ischemic brain regions without ischemic brain content of amino acid neurotransmitters, to explore the possible neuroprotective mechanism, and the Medical treatment of ischemic stroke to provide a reliable theoretical basis.Methods:35 cats were randomly divided into sham operation group, the local perfusion ischemia group, the cortex inhibitor group, the cortex stimulant group, PNS group, with middle cerebral artery cortical branch Steinmann puncture before the back to establish focal perfusion cerebral ischemia model, perfusion treatment group after 35 seconds to intravenous administration of saponins,.and inhibitors of propofol cortex, cortical stimulant caffeine as control observations removed after 5 minutes perfusion of cerebral tissue perfusion by liquid Tandem Mass Spectrometry amino acid neurotransmitters in brain tissue perfusion area of content, were statistically analyzed.Results:The perfusion of ischemic brain tissue ASP, GLU, GABA content (528.76±211.47μmol/ L,704.89±280.53μmol/L,345.82±168.12μmol/L) with the sham group (140.60±42.82μmol/ L,251.67±21.41μmol/L,137.40±35.76μmol/L) compared with significantly increased, with statistically significant (p<0.05), GLY levels between the two groups no significant difference (P> 0.05). PNS brain tissue GABA (518.97±170.9μmol/L) and perfusion ischemia group (345.82±168.12μmol/L) was significantly higher compared with a statistically significant (p<0.05), ASP, GLU, GLY content no significant difference between the two groups (p> 0.05). ASP, GLU, GABA, GLY content of propofol group, the caffeine group compared with the perfusion no significant difference in ischemic group (p>0.05). But the propofol group ASP, GLU content (497.97±194.95μmol/L,629.67±231.14μmol/L) and perfusion ischemia group (528.76±211.47μmol/L, 704.89±280.53μmol/L) had a lower.Conclusion:PNS can be applied to ischemic brain areas and not through a complex neural network, increase GABA content in ischemic brain areas, to provide after focal cerebral ischemia and neuroprotective effects, suggesting that Chinese medicine can not relieve the local ischemic brain areas ischemic brain injury, for the treatment of ischemic stroke in Chinese medicine to provide a strong basis. Propofol may be by acting on the ischemic brain areas not affected the release of excitatory amino acids and excitotoxicity play a neuroprotective role. The mechanism of action of saponins with propofol and direct regulation of neuronal excitability in varying degrees, with its unique mechanism of action.