Cerebral Hemorrhage, Axonal Regeneration Inhibitory Factor Receptor Ngr Expression And Axonal Injury

PartⅠPattern of NgR Expression in the Brain Tissue of Rats with Intracerebral Hemorrhage OBJECTIVE: The present study aimed to detect the expression of NgR in the brain tissue of rats with ICH.METHODS: 72 healthy male Wistar rats were divided into sham-operated group(n=36), ICH group(n=36). ICH model in rats were established as follows: after anaesthesia,100ul unclotted autologous blood was taken from the rats tail cut, then injected it with microsyine into internal capsule three times with stereotaxy. The rat brains were treated at 6h, 24h,48h, 72h, and 7d after operation . Immunohistochemical staining was used to examined the NgR expression . RESULTS AND CONCLUSION: A markedly increase of NgR expression was found at 6h in model groups compared with the sham controls( P< 0. 01), which peaked at 72h after the surgery ( P< 0. 01).CONCLUSION: The upregulation of NgR in the hemorrhage area might be an important feature of ICH and may play a role in the pathologic progression of this lesion.PartⅡStudy on Histomorphology andβ-APP Expression around Hematoma in Rats after Intracerebral HemorrhageOBJECTIVE: To study the dynamic change of histochemical morphologic and expression ofβ-APP at different post intracerebral hemorrhage intervals in rats.METHODS: 72 healthy male Wistar rats were divided into sham-operated group(n=36), ICH group(n=36).ICH model in rats were established as follows: after anaesthesia,100ul unclotted autologous blood was taken from the rats tail cut, then injected it with microsyine into internal capsule three times with stereotaxy. The rat brains were treated at 6h, 24h,48h, 72h, and 7d after operation.H&E Staining was used to show the histopathologic damage of perihematom.Immunohistochemical staining was used to examined theβ-APP expression around haematoma.RESULTS: (1) H&E Staining:In the sham groupThe brain tissue is normal, morphological features of the cell is intact in the sham group. Some necrotic neurons and several scattered inflammatory cells were observed in the perihematoma region at 6h after ICH. The inflammatory infiltration characterized by monocyte and necrosis increased markedly at 24 h, peak at the 72h. Meanwhile, gliosis and capillary hyperplasia appearing and around hematoma. The area of hematoma reduced markedly, accompanied by distinct glial and capillary hyperplasia at the 7 h d after ICH.(2) Compared with the sham controls .β- APP expression increased its immunoreactivity at 6 h, ( P< 0. 01) ,after 24h the expression ofβ- APP gradually increased, reached to maximize at 72h( P< 0. 01), and returned to basal level at 7 d, but still higher than that of sham groups. ( P< 0. 01)CONCLUSION: 1.Inflammatory infiltration, neuronal degeneration, neuron necrosis ,gial and Capillary hyperplasia in perihematoma are the features of experimental ICH rats. 2.With the increasing of inflammatory infiltration ,the expression ofβ-APP increased correspondingly .β-APP is time-dependently expressed in a pattern of up-regulation at early phase followed by down-regulation after ICH.β- APP can been used as a sensitive marker of axon damage after ICH.