Taohongsiwu Soup Microscopic Anastomosis In Rats Vascular Endothelial Repair And Vegf Expression

Objective:Observe micro the blood vessel hyperplasia interfering with the blood vessel inner film down in taohongsiwutang traditional Chinese medicine condition fitting and the effect expressing to VEFGMethod:With 30 3-month-old male rats weighing 200-250gSPF as the research object,①Experimental group:Taohongsiwutang fed;②control group 1:fed with saline; 30 rats were with 3% amyl Bobby duly sodium (1 ml/kg) line of peritoneal anesthesia, ten times the optical microscope in the anatomy of a rat's tail and cut off the middle of blood vessels; with 11 /0 non-invasive suture line after line of sutured allowed free movement, at 24 hours after,72 hours,168 hours animals were killed. Histopathological detection of vascular anastomosis under the cut-off for the fixed, embedded, sliced, HE staining and elastic fibers were observed under light microscope after the proliferation of the wall,the computer image analysis, the medial area and the ratio of observed Taohongsiwutang on neointima formation,and immunohistochemical detection of vascular endothelial growth factor (VEGF) to clarify the situation after endothelial repair.Result:When pink four things soups pour into 24 hours,72 hours and 168 hours being accustomed to an experimental group,the immune group spends VEGF expressing the power-up more obvious than brine collation group (equal P<0.05).Pink four things soups drink wine being convinced by group skill last 24 hours,form hyperplasia inner film comparatively normal saline contrast with each other 72 hours,168 hours increasing by set obviously (equal P<0.05),the normal saline raises inner film area and the ratio being hit by film area comparatively set obviously (.equal< P<0.05).