Adduction Combined With High-speed Counter-current Was Isolated Ligustrum Constituents And Pharmacological Studies

In this study, we usedβ-CD as the main elements and oleanolic acid (OLA) in Ligustrum lucidum as the Guest elements for inclusion, de-inclusion.Inclusion method combined with high-speed countercurrent chromatography (HSCCC) to separated OLA. While Water extract of L. lucidum was antibacterial experiment.β-CD as host molecules, OLA as guest molecules for inclusion, the inclusion complex was identified by X-ray with TLC. Methanol to de-inclusion. The results showed that:The electromagnetic stirring method, the inclusion ratio (nβ-CD: nraw materials) 2:1, inclusion temperature 70℃, inclusion time 1h, under the conditions could achieve to the best profit. TLC combined with X-ray could be identification of inclusion, the use of organic solvent extraction method could achieve better de-inclusion effect and OLA purity increased from 54.12% to 77.5%. Theβ-CD inclusion can play a certain purification effect on OLA.Sample 1 prepared by inclusion method and Sample 2 prepared by silica gel column chromatography were separated by HSCCC.We used TLC with HSCCC to select solvent system. Hexane:ethyl acetate:ethanol : water (16 : 14 : 14 : 5) as solvent system, the photogenic phase as stationary phase, the lower phase as mobile phase, HSCCC speed 900r/m, the separation time 400min, Distributed to the OLA purity of sample 1,2 were:93.8%, 96.4%.Inclusion method and HSCCC play a fine goal to separation of OLA.Water extract of L. lucidum for antibacterial activity, the use of test tube serial dilution method, we studied the L. lucidum decoction antimicrobial effect on Staphylococcus aureus, Escherichia coli and Bacillus subtilis. MIC of S. aureus was 31 mg/ml, MBC was 62 mg/ml, MIC of E. coli was 31 mg/ml, MBC was 62 mg/ml and MIC of B. subtilis was 4 mg/ml, MBC was 8 mg/ml. L. lucidum can inhibit the S. aureu, E. coli and B. subtilis.