Secondary Hyperparathyroidism In Patients Of Gata3, Th1/th2 Cytokine Changes And Dexamethasone Podocytes

Objective: To explore the possible mechanism and its clinical significance of changesin Th1/Th2 cytokine in ureamic patients with secondary hyperparathyroidism(SHPT).Method: Peripheral blood mononuclear cells (PBMCs) and serum were separatedfrom the blood of 30 uraemic patients with SHPT (iPTH>300pg/ml) as SHPT groupand 20 uraemic patients without SHPT(iPTH<150pg/ml) as non-SHPT group. Thetranscription levels of GATA3 gene was detected by semiquantitative reversetranscriptase-polymerase chain reaction (RT-PCR), Th1 (γ-IFN)/Th2 (IL-4) cytokinesbyELISA.Results: The transcription level of GATA3 in PBMCs in non-SHPT group was0.412±0.093 and that in SHPT group was 1.405±0.493. The transcription level ofGATA3 in patients with SHPT was significantly higher than that in non-SHPT group(p<0.01). The level of IL-4 andγ-IFN in serum in non-SHPT group was17.983±4.067pg/ml andγ-IFN 79.847±16.991pg/ml respectively; the level of IL-4andγ-IFN in SHPT group was 65.582±19.043pg/ml, and 69.842±9.75pg/mlrespectively. The level of Th2 cytokine(IL-4) and Th2 cytokine (γ-IFN) in SHPTgroup was significantlyhigher than that in non-SHPT group(P<0.01). In SHPT group,iPTH had significantly positive correlation with GATA3 (r2=0.8906, p<0.0001), andGATA3 also had positive correlation with IL-4 (r2=0.1854, p=0.0175).Conclusion: There are higher expression of GATA3 mRNA in PBMCs and the higher level of serum Th2 cytokine (IL-4) from uraemic patients with SHPT, furthermore,GATA3 has positive correlation with GATA3 and IL-4, respectively. These indicatethat uraemic patients may be in the immunodisturbance and GATA3 may play animportant role in the pathogenesis in uraemic patients with SHPT.Objective: To study the effects of different concentration dexamethasone on GATA3and th1/th2 of podocytes and the possible mechanisms involved.Methods: Experiments were performed using growth-restricted, conditionallyimmortalized human podocytes, these podocytes were respectively treated withdifferent concentration dexamethasone (10-3,10-5,10-7,10-9,10-11M) for 12h,24h ,48hand 96h , The transcription levels of GATA3 gene was detected by semiquantitativereverse transcriptase-polymerase chain reaction (RT-PCR), Th1(γ-IFN)/Th2(IL-4)cytokine byELISA.Results: dexamethasone inhibits the expression of GATA3 mRNA in both time anddose depended manners; on the contrary, as the lower concentration ofdexamethasone ,the expression of GATA3 has relatively higher, simultaneously,TH2cytokine(IL-4) higher,TH1 cytokine(γ-IFN) lower.Conclusion: The treatment of dexamethasone of podocyte disease is through GATA3and TH2 cytokine ,which these indicate GATA3, Th2 cytokine mayplayan important role in the pathogenesis of these disease.