Xuebijing On The Role Of Acute Pulmonary Embolism Protection

Objective To study the expression levels of endothelin-1(ET-1)in lung vascular endothelium,bronchial and alveolar epithelia ,thromboxane A₂(TAX₂) and prostacyclin (PGI₂) in plasma and arterial blood gas in acute pulmonary thromboembolism(APTE) of rabbit,and explore the effects of thrombolytic( urokinase,UK)and ant-inflammatory therapy(xuebijing)on their expressions.Methods Thirty rabbits were randomly divided into the control group,the pulmonary thromboembolism(PTE)model group,the Xuebijing therapy group,the UK therapy group and an UK + Xuebijing therapy group,with 6 rabbits in each group. The PTE model was established by intravenous injection of autologous blood clots. Arterial bloodgas were analyzed, and Plasma's concentrations of TXA₂ and PGI₂ by were examined by radioimmunoassay before and 1h ,2h,4h,8h after injection. Pathological changes of the lung were examined with light microscope, and the expression levels of ET-1 in lung vascularendothelium,bronchial and alveolar epithelia were examined by immunohistochemistry.Results:①Arterial blood gas analysis showed that PO₂ and PCO₂ were obviously lower in PTE model group than the control group ,no significance found in Xuebijing group and PTE group;the PO₂ improved earlier in UK and UK+Xuebijing groups than in PTE group.②radioimmunoassay showed that TXA₂ and PGI₂ in the PTE group increased at 1h ,peaked at 2h,decreased at 4h ,which also were higher than those in the control group at 1h,2h,4h(P<0.01),In the treat groupTXA₂ andPGI₂ increased at 1h,peaked 2h ,decreased after 2h ,also decreased fasted than those in PTE group at 1h,2h and 4h irrespectively(P<0.01), especially in UK+Xuebijing group . There were no differences in TXA₂ andPGI₂ before injection and 8h after injectionin in each groups.③Histopathological study showed that lung injury was obvious in the PTE,but was less inUK and Xuebijing groups ,and least inthe UK + Xuebijing group.④the immunohistochemistry results showed in the PTE and Xuebijing groups,the expression levels of ET-1 in lung vascular endothelium,bronchial and alveolar epithelia were significantly higher compared those in the control group . The UK and UK+Xuebijing groups were significantly lower in ET-1 as compared to the PTE group.Conclusion: After APTE,thrombolytic and anti-inflammatory treatment could improve arterial blood gas ,decrease TXA₂,PGI₂ and acute lung injury induced by ET-1. Thus,Xuebijing injection may alleviate pulmonary inflammation and decrease acute lung injury in APTE . Anti-inflammatory therapy should be considered in APTE when thrombolytic treatment was used.