Contribution Of Rankl-rank-opg In The Osteolytic Lesion Around Hydatidocystis Which Parasitize Spine Of Meriones Meridianus

Objective: To explore the expression of RANKL/RANK/OPG and TNF-αin the osteolytic lesion around hydatidocystis which parasitize bone of Meriones meridianus spinal hydatid disease models, and observe the rang of the expression during a certain period Methods: To chose randomly 100 healthy fomale meriones meridianus with the age of 2~3 months and the weight of (40±5)g to establish the spinal hydatid animal models ,with 60 same ones as controls. Six months later, choose 45 spinal hydatid disease models and 45 controls, then divide them into two 3 groups by disease and time point, each group content is15 mice. To kill respectively a group of models and a group of controls after 0,1,2months, then measure the expression of TNF-αby immunohistochemistry and RANKL, RANK, OPG by in situ hybridization in the osteolytic lesion around hydatidocystis which parasitize bone of Meriones meridianus spinal hydatid disease models and in the cancellated bone of controls. Results: The model of spinal hydatid was succesfully established,and the number of models can content the experiments. Comparing with the controls, the expression of RANKL, RANK, TNF-αin the osteolytic lesion around hydatidocystis which parasitize bone of Meriones meridianus spinal hydatid disease models is significant (P<0.05), while the expression of OPG is declining. And there are no differences among the same models at the different time. Conclusion: TNF-α, RANKL, RANK make their contribution in the osteolytic lesion around hydatidocystis which parasitize spine of spinal hydatid disease models,while OPG prevent the cases from taking. However,the measure of them is not relative to the period of the disease models.