| Objective:To observe the spleen Spleen Deficiency Rats cytokine interleukin-2 (IL-2), interleukin-6 (IL-6), tumor necrosis factor (TNF-α) and spleen weight index of.Methods:60 female rats, using randomly divided into four groups:normal control group, spleen model group, low dose group, Chinese high-dose group, 15 rats in each group. Reserpine subcutaneous injection method of producing animal models of spleen, spleen weight measured by electronic balance, measured by radioimmunoassay IL-2, IL-6 and TNF-a and so on.Results:1. Modeling days before and after the increase in body weight of rats compared with model group and normal group showed significant (P< 0.01), suggest the modeling is successful.2. Model group of spleen weight index smaller than the other three groups, the difference was significant (P<0.05). TCM treatment group compared with the control group there were significant differences (P< 0.05).3. Compared with model group, traditional Chinese medicine group of IL-2, IL-6, TNF-αlevels were higher than the untreated group, the difference was significant (P<0.01), model group and control group were significant differences (P< 0.05).4. TCM treatment group low dose and high dose group between the two groups spleen weight index, IL-2, IL-6, TNF-αwere not significantly different (P<0.05). Conclusion:The results show that the rat spleen produce IL-2, IL-6, TNF-αand other cytokines decreased ability to lower immune function in the state, strengthening the spleen can Treated rats the spleen index, spleen, IL-2, IL-6, TNF-αincreased to adjust the body's immune function. |
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