| Asthma is a common disease, approximately more than 100 million patients worldwide. In China, the incidence of asthma is about 1% to 4%,worldwide incidence of asthma was increasing trend year by year. As the disease mechanism has not yet been fully elucidated, prevention and treatment of asthma is very difficult. Asthma is the eosinophils (eosinophil, EOS), mast cells and T lymphocytes and other inflammatory cells involved in chronic airway inflammation. Total serum IgE levels closely related to the development of asthma, IgE-mediated asthma reaction speed of onset, trigger asthma inflammation cascade.Asthma in recent years has been that there is Th1/Th2 imbalance in the body, Th2 type cytokines IL-4, IL-5, IL-13etc can be controlled in B cells to produce IgE, the promotion of bone marrow progenitor cells differentiate to eosinophil chemotactic to airway. In airway hypersecretion and airway hyperresponsiveness play an important role. B cells were synthesized IgE. The Aiolos and Ikaros mainly T cells and B cells regulate the development, balance and proliferation, Aiolos may be involved in the pathogenesis of asthma by lymphocytes.Glucocorticoids are currently the most effective anti-inflammatory drugs, through multiple links asthmatic response and reduce airway hyperresponsiveness. Currently at home and abroad have not seen yet glucocorticoid on lung tissue in asthmatic Aiolos change reports, this study was to establish the asthmatic model in rats was observed in lung tissue of asthmatic rats and gene expression of Aiolos and pathological changes in lung function, explore the mechanism of dexamethasone in treatment of asthma.MethodsSPF level obtained SD 30 rats were randomly divided into 3 groups of 10 each. Prepared by means of reference to Petersen LC asthma model. Model group:the first day of sensitization by intraperitoneal injection of 10% of the chicken ovalbumin (OVA) and 10% of the mixture of aluminum hydroxide 1ml, the same dose on day 8, the same method to enhance allergen 1; sensitized after 15 days, the rats were placed in a sealed glass container of 1% chicken ovalbumin inhalation stimulation (fog particle diameter of 3-5μm), placed in the fog chamber for about 30 minutes/day, one day inspire for 2 weeks. Dexamethasone group:In the asthma group, based on the inhalation of 1%VOA ago 1h,Pre-peritoneal injection of dexamethasone2mg/kg. Control group:they were given by normal saline injection and inhalation, and the remaining steps in the same model group.24 hours after the last challenge, the rats were anesthetized, killed by blood letting. To take blood 2mL, using EDTA anticoagulant, using automatic blood cell count measured EOS. The rat's right lung tissue for pathological testing. Extraction cell, RT-PCR detected Aiolos mRNA expression.Statistical Methods:Data presented as mean±standard deviation (x±s), said statistical analysis of all data to SPSS11.0. Multi-group comparison of mean variance analysis F test was used to compare two groups, q test and correlation analysis using Spearman rank correlation analysis. P<0.05 indicated significant difference.Result:Lung tissue of normal control group detected no significant changes in airway inflammation. Bronchial asthma can be seen around the large amount of inflammatory cell infiltration, including macrophages, lymphocytes, neutrophils and EOS, etc.; inflammatory cells primarily in the bronchial submucosa and mucosa of the outer layer around small blood vessels and alveolar infiltration, etc.; see trachea stenosis, airway epithelial cell loss, significantly increased airway secretions, bronchial wall thickening. Dexamethasone group, the small airway wall thickening, increased secretions, alveolar wall thickening and inflammatory cell infiltration significantly reduced compared with asthma group.Asthmatic peripheral blood leukocytes and eosinophil percentage with the control group and dexamethasone group were significantly higher, the difference was statistically significant (total number of leukocytes and dexamethasone group P< 0.05, the proportion of eosinophils and dexamethasone group P<0.01, both compared with the control group were P<0.01). WBC normal control group and dexamethasone group compared with the dexamethasone group, no difference P< 0.05, the proportion of eosinophils compared with the dexamethasone group there was significant difference P<0.01.Lymphocytes AiolosmRNA rats were expressed. Normal control group expression in rat lymphocytes AiolosmRNA more. Asthma group significantly decreased the expression of lymphocyte AiolosmRNA. And normal control groups were significantly different (P<0.05); Dexamethasone expression in rat lymphocytes AiolosmRNA D examethasone group with varying degrees of increased (P<0.05), but slightly lower than the normal control group (P<0.05).Conclusion1. Asthmatic rats Aiolos gene expression was decreased,2. Dexamethasone increased Aiolos gene expression in asthmatic rats,3. Dexamethasone can improve airway inflammation in asthmatic rats. |
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