| Backgrund and objectiveOriginal liver cancer is the one of common malignant tumor of our country, hepatocellular carcinoma ( HCC) takes the 91.5% of orginal liver cancer, according to the investigation of malignant tumor death rates, the lethal rates of origina liver cancer is the position of 3th and of 4th in male and female malignant tumor respectively. The occurence of tumor is a long-term,advance gradually,the complex process,multistage part and many steps, cell hyperplasias and the disorderof regulation is the one of common feature of all malignant tumors.Livin gene as inhibitor of apoptosis protein( IAP ) in clan,the structure of LAP that had been discovered are unique, protecting specific cell resistance a series of stimulate original guide, it inhibitors apoptosis of cell , therefore, Livin gene is consider as a kind of new restrain cell to wither deceased cancer gene, the prognosis and the malignant level of various tumor are related closely, such as, lung cancer, bladder cancer, cancer of the stomach and nose pharynx cancer etc. however, it is very few to look up the domestic and international document its related research in hepatocellular carcinomaβ-catenin are a kind of important afterbirth protein, it has double function, one is situated between E- calcium , its exception changes in tumor to soak , transfer process in play important role; second is the crucial signal member of WNT ,its signal conduct channel, degradation obstacle causes afterbirth quality dissociateβ-catenin accumulation , and strengthen factor( TCF/LEF) combination with T cell factor / lymph cell to enter nucleus , activation the lower target gene c-myc transcribe , it is out of control to arouse cell to hyperplasia and break up , cause tumor to occur, nowdays, the research for hepatocellular carcinoma showsβ-catenin genetic mutation and over expressssion with tumor occur prognosis to be related closely, the unusual activation of the signal route ofβ-catenin will be the prospective new channel that becomes the way of treatment of liver cancer, this gene expresses with tumor pathology grade and the relation of prognosis have quite many disputes at present,The research shows that prognosis of tumor is good to be related.Studying to adopt immunity systematization the detection of Livin,β-catenin at hepatocellular carcinoma and near cancer " normal" organization in expression, at the same time ,analysing between two genes express have correlation, in order to get the reason that discussion hepatocellular carcinoma occurs , to seek the gene treatment of liver cancer, offering theory and test basis.Materials and methods1. Collecting Zhengzhou university first affiliated hospital ( 1996-2006) file wax pieces of pathology department after amputated because of the operation of liver cancer. Select 40 regular hepatocellular carcinoma and 10 regular cancers near normal liver organization, in which, 18 examplies of high break up , medium break up is 12 examples and 10 low break up ; Near cancer, the 16 transfered examples and lymph node of lymph node do not shift 24 examples, the organization specimens socked into the neutral medical liquid , after conventional dehydration.2. Applicating immunity systematization ( SP law) studies to detect Livin andβ-catenin near hepatocellular carcinoma and cancer normal organization in expression condition and analysis.3. All datas of experiment are handled with SPSS10.0 statistical analysis software, the comparison of two sample rates is related with the inspection of X~2 of the rate of across block to analyse with Spearman grade related analysis. Take a=0.05 to inspect level.Results1 .Livin in the expression of HCC organizationthe positive dyeing of Livin in hepatocellular carcinoma is palm yellow pellet, position in cell thick liquid. Livin in the result of liver organization expression shows in Table 1.high expression of Livin in the organization of liver cancer, there are 31 regular positives( expression rates 77.50%) in 40 examples, in 10 regular normal organizations has 2 examples are weak expression ( expression rates 20.00%), 8 regular negative and the discrepancy of 2 groups has statistics meaning( P < 0.05); the expression of Livin with cancer organization broken up relevant, low break up group and high break up group have discrepancy(p < 0.05),high break up group and medium break up group .medium break up group and low break up have no statistics meaning(p > 0.05).12 examples Livin in the 16 regular transfered of lymph node of liver cancer expressed positive( expressssion rates 75.00%), no shifted group with lymph node to have no statistics discrepancy ( p < 0.05).2.β-cateninin HCC the expression of organization the positive dyeing ofβ-catenin in hepatocellular carcinoma is palm yellow pellet, position in cell thick liquid .β-catenin in the result of liver organization expression shows in Table 1.high expression ofβ-catenin in the organization of liver cancer, there are 29 regular positives( expression rates 72.50%) in 40 examples, in 10 regular normal organizations has 3 examples are weak expression ( expression rates 30.00%), 8 regular negative of 10 normal liver cells , discrepancy of two groups has statistics meaning( P < 0.05);the expression ofβ-catenin with cancer organization broken up relevant, low break up group and high break up group have discrepancy(p < 0.05),high break up group and medium break up group ,medium break up group and low break up have no statistics meaning( p > 0.05).8 examplesβ-catenin in the 16 regular transfered of lymph node of liver cancer expressed positive( expressssion rates 87.50%), no shifted group with lymphnode to have no statistics discrepancy(p < 0.05).3 The relation of expression of Livin andβ-cateninthe expression ofβ-catenin and Livin hepatocellular carcinoma 1 the positive number together is 27 examples, it is 7 examples to negative expression together,β-catenin of the Livin gene positive in the liver cell cancer ornization that expresses negative is 4 examples, the positive ofβ-catenin has 2 regular Livin negatives expressed , with spearman grade related analysis inspection, two grous obvious positive (r=0.826 P < 0.05) result.Conclusions1.High expression of Livin in HCC organization, and with histology grade isconcerning the transfering of lymph node, so, Livin is possible with theoccurrence,development and prognosis of liver cancer to be related , and withLivin gene, carrying out gene treatment to offer theoretical basis for target point.2.High expression ofβ-catenin hepatocellular carcinoma organization and normalliver organization has significance discrepancy, showing positive expression ofβ-catenin with liver cancer occurs relevantly, at the same time also grade withhistology relevant.3.The expression of Livin andβ-catenin show obviousiy positive co- relation ,alsoshow them possiblely when HCC occurs and develops in takeing mutuallycoordinative role.4. The relation of expression of Livin andβ-catenin with HCC organization to be broken up level and transfermation of lymph node will enlarge sample number to be studied further. |
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