Correlation Of Collagen Type â…¡ C-telopeptide (ctx-â…¡) To Intervertebral Disc Degeneration And Osteoarthritis

BackgroundCollagen typeⅡis the major structural component of articular cartilage. It is also the major component of terminal plate and nucleus gelatinosus. Collagen typeⅡC-telopeptide (CTX-Ⅱ) is the split product of collagen typeⅡ. It only generated from ripe structural collagen and ingressed into urine as a small peptide form. Investigation has revealed that the content of urinary CTX-Ⅱin osteoarthritis (OA) patients are higher than health adults. But the relationship between the content of urinary CTX-Ⅱand intervertebral disc degeneration (IVDD) has not reported in the domestic literature yet.ObjectivesTo study the correlation of collagen typeⅡC-telopeptide (CTX-Ⅱ) to intervertebral disc degeneration and osteoarthritis, and to investigate the difference of CTX-Ⅱin the urine of the patient with intervertebral disc degeneration and osteoarthritis.MethodsFifteen patients with intervertebral disc degeneration, including 7 male and 8 female, with mean age of (62±7) years old, and 15 patients with osteoarthritis, containing 6 male and 9 female with mean age of (61±8) years old were selected. In addition, 15 patients (8 male and 7 female) without intervertebral disc degeneration or osteoarthritis, with mean age of (57±6) years old were served as the control group. For each patient, fasting second-void morning urine samples were collected and stored at -20℃. After all the samples were collected, urinary CTX-Ⅱwas measured by enzyme-link immunosorbent assay. Meanwhile, the creatinine concentrations of each sample was detected by creatinine kit for correct the CTX-Ⅱ. All the data were analyzed with SPSS10.0 statistical software. The correction value of CTX-Ⅱwith 3 groups of patients were analyzed by one way analysis of variance after test of normality and homogeneity test for variance. Meanwhile, the content of urinary CTX-Ⅱin intervertebral disc degeneration and osteoarthritis groups were analyzed with the grade of degeneration by regression analysis. P<0.05 was considered the level of significance.ResultA total of 45 patients were included in the final analysis. Compared with the control group, the content of urinary CTX-Ⅱin intervertebral disc degeneration and osteoarthritis groups were higher, and the distinction had statistical significance (P < 0.05). Compared the content of urinary CTX-Ⅱbetween intervertebral disc degeneration group and osteoarthritis group, the distinction had no statistical significance (P > 0.05). There was positive correlation of urinary CTX-Ⅱto the grade of intervertebral disc degeneration and osteoarthritis (r=0.592,0.719;P=0.020,0.003).ConclusionsThe content of urinary CTX-Ⅱstep up both in intervertebral disc degeneration patients and osteoarthritis patients. Urinary CTX-Ⅱhas direct correlation with intervertebral disc degeneration and osteoarthritis.