| Objective: To investigate the efficiency of bone repair in rat calvarial defects by Biomimetic hydroxyapatite-collagan complex carrier/BMP2-derived peptide P24 system, and the preliminary comparison of bioactivity between bmp2-derived peptide P24 and rhBMP2Methods: Five-millimeter critical-size calvarial defects were created in 30 male Sprague–Dawley rats. The animals were divided into three groups of 10 animals each. The defects were treated with PLGA-NHAC (A group), rhBMP-2/PLGA-NHAC (B group), P24/PLGA-NHAC (C group). Defects were evaluated by X-ray, radiodensity, CT scan, histology, following a 6- and 12-week healing interval (5 animals/group/healing intervals).Results:①Radiological examination: The percentages of the regenerated areas in A group were significantly lower than B group and C group at 12 weeks (α<0.05),but no significant difference between B group and C group(α>0.05).The comparison of the gray levels also shows the difference.②Histological examination: At 6 weeks post-surgery, Small amount of biomimetic material in group A degraded, free of inflammation; and B group and C group show a large number of new bone tissue and osteoblasts. At 12 weeks, the defect sites were filled with dense connective tissue and small particles of residual PLGA-NHAC, minimal new bone formation was observed in group A. The quantity of the newly formed bone was greater than that observed at 6 weeks in B and C group, and the bone showed more advanced stages of remodeling and consolidation.Conclusion: P24 peptide is with strong osteoinductive activity and stability in vivo, and PLGA-NHAC is an ideal scaffold and a sustained release carrier. It is suggested that P24/PLGA-NHAC system may be a available matrix material for clinical bone defect. |
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