The Study Of Correlations Between Eb Virus Infection And Survivin Expression In Han And Uyghur Nasopharyngeal Carcinoma In Xin Jiang

Objective:To detect the expression of EB virus in nasopharyngeal carcinoma, to detect the expression of Survivin,Ki-67 in nasopharyngeal carcinoma. To investigate the relationship of EB virus infection and Survivin expression and Han, Uygur nasopharyngeal carcinoma.Methods:Selected 135 cases of nasopharyngeal carcinoma, 50 cases of nasopharyngeal chronic inflammation as control. Envision two-step detection of EB virus infection in nasopharyngeal carcinoma.,of negative cases by immunohistochemistry and then detected the expression of EBER by in situ hybridization, comparison of the sensitivity of the two detection methods.Results:(1) LMP1-positive rate was 65% (88/135) detection of immunohistochemical, much higher than in chronic inflammation of nasopharyngeal 18%(9/50), and the difference has statistical significance (P<0.05). (2)The expression of LMP1 different ages, gender, ethnicity, histological type in nasopharyngeal carcinoma no statistical significance (P>0.05); LMP1-positive rate lymph node metastasis group was higher than that without lymph node metastasis group, the difference has statistical significance (P<0.05). (3)47 cases of LMP1 negative immunohistochemical was detected by in situ hybridizationthe the expression of EBER, 38 cases of positive results, the joint detection of two methods to improve the accuracy of detection. (4)The expression of Survivin,Ki-67 was higher in nasopharyngeal carcinoma than in chronic inflammation of nasopharyngeal,and the difference has statistical significance (P<0.05). (5)The expression of Survivin different ages, gender, ethnicity, histological type in nasopharyngeal carcinoma no significant difference (P>0.05), lymph node metastasis group and without lymph node metastasis group no significant difference (P>0.05).Conclusion:(1) The relation of nasopharyngeal carcinoma and EBV infection are closely,LMP1 may be involved in malignant transformation and tumor invasion and metastasis of cancer cells; EBV infection no significant difference in the Han,Uygur nasopharyngeal carcinoma. (2) Immunohistochemical detection of EBV infection can be used as a screening method, but EBER in situ hybridization detection of EBV infection is more sensitive, and may improve the diagnostic rate, the two methods can be combined to improve the accuracy of detection, thereby reducing the false opportunities for negative. (3) The expression of Survivin,Ki-67 much higher in nasopharyngeal carcinoma than in chronic inflammation of nasopharyngeal, indicating that the occurrence of nasopharyngeal cancer may be related to inhibition of apoptosis, proliferation-related out-of-control, the relationship between Survivin and lymph node metastasis of nasopharyngeal carcinoma need further study.