The Meaning Of Diagnosis About Tcr And Igh Gene Rearrangements In T-lymphocyte Infiltration With Epidermotropism Disease

Objective:Specific TCR and IgH gene rearrangements in the diagnosis of mycosis fungoides importance and to explore the identification of TCR and IgH gene rearrangements in pro-pathology of skin diseases as the significance of mycosis fungoides discussed the development of psoriasis and vice correlation.Methods:Using phenol-chloroform method Xinjiang Uygur Autonomous Region People's Hospital, Department of Dermatology collected 17 cases of confirmed mycosis fungoides,32 cases of suspected mycosis fungoides,10 cases and 10 cases of eczema, psoriasis, Vice paraffin embedded tissue for DNA pumping mentioned, and PCR amplification using the polymerase chain mycosis fungoides, psoriasis and eczema gene deputy.Results:(1) 17 cases diagnosed as mycosis fungoides in nine cases of TCR gene rearrangement was positive, the positive rate was 52.94%; IgH gene rearrangement in 5 cases, the positive rate was 29.41%; (2) 32 cases of suspected mushroom granuloma in two cases of TCR gene rearrangement was positive, the positive rate was 6.25%; IgH gene rearrangement in 14 cases, the positive rate was 43.75%; (3) 10 cases of psoriasis of the TCR vice gene rearrangements were negative; four cases of IgH gene rearrangement, the positive rate was 40%. (4) 10 patients with eczema TCR gene rearrangement and IgH gene rearrangements were negative.Conclusion:(1) 17 patients with histopathological diagnosis of mycosis fungoides TCR gene rearrangement positive in 9 cases, the positive rate was 52.94%, has a great value in diagnosis and determine the cell type of origin. (2) suspected of mycosis fungoides in 32 patients with two cases of TCR gene rearrangements, the positive rate was 6.25%,14 patients had a positive IgH gene rearrangement, can be used in the diagnosis and identified from a suspected malignant lymph tumor, follow-up more important. (3) Deputy psoriasis 4 of which were positive for IgH gene rearrangement, this result can be overlapping pollution in experiment and follow-up more important.(4) gene rearrangement can distinguish benign and malignant lymphoma of the important means.