Study On The Significance Of Expression Of Ho-co System In Diabetic Foot Patients

Objective:To explore the change of HO-CO system in diabetic foot patients, in order to investigate its contribution to diabetic foot.Methods:(1)Sample collection:the serum and foot skin of diabetes foot patients (30 cases), diabetes patients (25 cases) and control (25 cases) ware collected. At the same time, the clinical data such as age, sex, course of disease were recorded. (2)Detection of the samples:Chalmers'way was used to detect the serum CO; The immunohistochemistry was used to obverse the expressions of HO-1, HO-2.(3)Statistical analysis:The SPSS 11.0 was used for Statistical analysis. the results were expressed as means±SD (x±s). The statistical analysis of the data were performed using analysis of variance (ANOVA). A p value of less than 0.05 was considered statistically significant.Results:(1)HO-CO system Serum CO:the serum CO of the control group, the diabetes group and the diabetic foot were 1.021±0.192,0.860±0.199,0.807±0.148 mg/L, difference between patients diabetics(including diabetes group and diabetic foot group) and control is statically significant(P<0.05). But there is no difference between diabetes group and diabetic foot group (P>0.05).(2)Expression of HO-1:the expression of HO-1 in each group is statically different (P<0.05). In diabetes group, the expression is weaker than in control. The expression in diabetic foot is the weakest among the tree groups (P<0.05).(3)Expression of HO-2:the expression of HO-2 in each group is statically different (P<0.05). In diabetes group, the expression is weaker than in control. The expression in diabetic foot is the weakest among the tree groups (P<0.05).(4)Correlation and regression analysis:there is significant correlation between serum CO concentration and the expression of HO-1 and HO-2 in foot skin.(P<0.05), and the relationship between serum CO concentration and the expression of HO-2 is more intimate.Conclusion:(1)The serum CO concentration in diabetes patients may be decreased in the early stage, but it changes lowly in the later stage. The expression of HO-1 and HO-2 in foot skin tissue is significantly decreased, especially for diabetic foot patients.It indicates that the decreased expression of HO-CO system may participate the occurrence of DF.(2)HO-CO system may play an important role in the occurrence and development of diabetic foot.