Experimental Study On The Treatment Of Endotoximia By Hemoperfusion With Hb-h-7 Resin Absorption In Dogs

OBJECTIVE:Discuss the effectiveness and safety of treating the endotoxemia dog model with hemoperfusion application of HB-H-7 resin.METHODS:12 dogs were divided randomly into treatment and control groups, 6 dogs in each group. Endotoxemia of model dogs was performed by infusing endotoxin 0.5 mg/kg through central venous using micro-infusion pump. HB-H-7 resin, which was prepared by projective preparation and high-pressure sterilization, perfused for 2h through whole blood hemoperfusion.RESULT:The plasma endotoxin levels of treatment group were significantly decreased (p<0.05) after hemoperfusion, otherwise, there was no significant decline in the control group (p>0.05), so there were significant difference (P<0.05) between the two groups. Plasma protein was affected by HB-H-7 resin in some degree. Total plasma protein declined 25.9% after perfusion, in which plasma albumin fell 18.2%, and globulin 34.2%, there was significant difference(p<0.05) in statistics. There was no apparent impact on the red blood cells, platelets and electrolyte. All of the model dogs in treatment group survived healthy for a long time after experiment, but all of that in the control group were died after 14-22 h.CONCLUSION:Endotoxin can be highly absorpted by HB-H-7 resin, so it is safe and effective to treat endotoxemia dogs animal model with HB-H-7 resin through hemoperfusion.