Effects Of 17α-estradiol On The Aβ Synthesis Of App/ps1 N₂a Gene Transgenic Cells

Objective To investigate the effects of 17a-estradiol (E2a) on the release ofβ-amyloid protein (Aβ) with the transgenic cell line APP/PSl N2a, and discuss the potential mechanism of such effects.Methods APP/PSl N2a cell lines were pretreated with 10 nM E2a for 2 days, then changed the culture medium, and treated with different methods:(1) cell lines were treated with either E2a (25nM,50nM, 100nM,200nM) or vehicle, The protein level of Aβand BACE1 both intracellular and extracellular of APP/PS 1 N2a cells were detected by Western blot. (2) cell lines were treated with 100nM E2p,20 mM glucose and 5 mU glucose oxidase(GOX), followed by exposure to the glucose oxidase (G/Gox) to induce oxidative stress. After 24 hours, the change of the ROS contents in APP/PS 1 N2a cells was observed for DHE.Results Pretreatment of APP/PSl N2a cells with different concentration (25nM,50nM, 100nM,200nM) E2a significantly reduced extracellular levels of Aβ(P<0.05). There was no change both in the intracellular levels of Aβand the levels of BACEl(P>0.05). E2a can reduce the ROS produced by APP/PS 1 N2a cells after G/Gox treatment.Conclusion E2a can reduce the production of extracellular Aβ, its mechanism may be related to the oxidation damage.