| Objective:To investigate the different oxygen methods in order to explore the damage of fetal rats intrauterine ischemia anoxia reperfusion kidney.。Methods:A total of 60 pregnant SD rats were randomly divided into 3 groups, abnormal group (A group), Virtual operation group (B group), hypoxia-ischemia reperfusion group (C group), which was divided into four group:non-oxygen group (C1), low concentration of intermittent ventilation group (C2), low concentration pulse oxygen group (C3), low-level persistent oxygen group (C4), n= 10. A group of pregnancies without any treatment; B group no uterine artery occlusion, line switches abdominal treatment; C group when pregnant 17 days were cope with uterine artery occlusion for 15 minutes completely and then restore the blood supply, C1 group no oxygen, C2, C3, C4 group were given oxygen intervention with different ways when 18 days pregnancy, and the oxygen concentration was 37%:C2 group oxygen 30min time of three times a day, C3 group oxygen 30min, stop 5min, and then 30min with three times per day, C4 group were provided 8 hours continuous oxygen. When pregnancy 21 days all pregnant rats were cesarean section, calculating the survival rate of fetai mice in each group and weighing, were decapitated rapidly,acquired fetal sacrificing litter fetal blood urea nitrogen and creatinine testing, application Stained by HE groups fetal renal pathological changes and to observat immunohistochemistry of renal expression of intercellular adhesion molecule-1.Results:(1) The rate of fetal survival and weight:the fetal survival rate of all the group was high, but the difference has not statistically significant. Comparison of average body weight of each group:A group (4.21±0.22) g and the B group (4.24±0.31) g difference has not statistically significant (P> 0.05); C1 group (3.65±0.24) g were significantly lower than group B (P<0.05); C2 group (4.30±0.21) g and the C3 group (4.23±0.18) g higher than the C1 group and the C4 group (3.56±0.20) g (P <0.05), and the B group showed no significant difference (P> 0.05), C2 and C3 was no significant difference between (P> 0.05); C4 average body weight less than the C1 group (P<0.05).(2) Pathological changes in fetal rat kidney:the group of A, B,C2 and C3 with normal renal structure, hierarchy and tidy, darker and thinner cortex. A lots of renal corpuscles was observed on the capillary section; vitro renal cyst wall was formed with Renal Week simple squamous epithelium.no exudation and hemorrhage within the cysts. Only a partial renal tubular epithelial cell were found degeneration. C1 renal tissue degeneration, some of the small tube flat brush border epithelial cells, shrinkage, loss, nuclear deeply stained, bare, severe cell necrosis on site see, shedding, basement membrane is incomplete; a part of the tubular lumen expansion Some see the debris and tubular epithelial cells, cells see. interstitial infiltration of inflammatory. C4 groug kidney degeneration and some tubular epitheli.al cell brush border flat, shrinkage, loss, nuclear stained,and which was similar.with the C1 group。(3) Fetal renal tubular injury score:A group (3.16±1.05) and B group (3.84±0.75) was no significant difference; C1 group (37.11±6.73) were significantly higher than the B group (3.84±0.75) (P<0.05);the group of C2 (11.57±4.40) and C3 (14.34±5.95) significantly lower than C1 and C4 group (43.42±7.88) was (P<0.05), but significantly higher than B group (P<0.05), there were no significant difference between group C2 and C3 (P> 0.05); C4 was significantly higher than the Cl group (P <0.05)。(4) The expression of intercellular adhesion molecule-1 in fetal renal:A group (131.22±5.93) and group B (130.61±4.71) compared no significant (p> 0.05); C1 group (118.24±7.08) was significantly lower than B (130.61±4.71) (P<0.05); the group of C2(127.21±7.06) and C3(124.13±5.87) significantly higher than the group of C1 and C4 (111.74±7.71)(P<0.05), but compared with B group no significant difference (P> 0.05), while C2 and C3 no significant difference between the other groups (P> 0.05); and C4 was significantly higher than C1 group (P<0.05)(5) Test renal function:BUN:A group (9.93±0.69) mmol/L compared with the B group (9.66±1.01) mmol/L has no significant difference (P> 0.05); C1 group (22.08±3.24) mmol/L was significantly higher in the B group;the group of C2 (12.62±2.26) mmol/L and C3 (14.31±2.78) mmol/L significantly lower than the group C1 and C4(25.47±4.17) was (P<0.05), but higher than the B group(P <0.05); The difference between C2 group and C3 group has not statistically significant(P>0.05); C4 group was significantly higher than the Cl group (P<0.05). Cr:Compared the A group (130.31±5.28) umol/L and the B group (132.26±4.11) umol/L,there were no significant difference (P> 0.05); C1 group (158.34±7.72) umol/L was significantly higher than B; The group of C2 (139.19±6.56) umol/L and C3 (144.13±5.93) umol/L was significantly lower than the group C1 and C4(171.58±8.74) umol/L (P<0.05), but higher than the B group (P<0.05); Compared C2 and C3 group was not statistically significant (P> 0.05); C4 was significantly higher than the C1 group (P<0.05)。Conclusion:(1) Ischemia-reperfusion injury maybe cause fetal weight loss of the fetal mice, suggesting that fetal chronic hypoxia cause fetal growth restriction.。(2) Hypoxia-ischemia-reperfusion could lead to renal injury of fetal mice。(3) Low of continuous oxygen and low density of pulse oxygen can reduce fetal intrauterine ischemia-reperfusion renal injury, however sustaining low concentrations of oxygen may aggravate the kidney damage。... |
PDF Full Text Request